High-throughput sequencing (HTS) data of plasmid library subjected to Cas12a cleavage Karthik Murugan Dipali Sashital Arun Seetharam Andrew Severin 10.25380/iastate.8178938.v3 https://iastate.figshare.com/articles/dataset/High-throughput_sequencing_HTS_data_of_plasmid_library_subjected_to_Cas12a_cleavage/8178938 <div><p>Cas12a (formerly Cpf1) is an RNA-guided endonuclease in the CRISPR-Cas immune system that can be easily programmed for genome editing. To understand the native cleavage specificity and cleavage activity of Cas12a, we employed high-throughput <i>in vitro</i> cleavage assays using a plasmid library (pLibrary). We tested the cleavage activity of three Cas12a orthologs – FnCas12a, LbCas12a and AsCas12a. For each Cas12a ortholog, we used three different crRNA sequences and corresponding target libraries (pLibrary), referred to as pLibrary PS4, EMX1 and CCR5. The data set is the high-throughput sequencing (HTS) reads of pLibrary subjected to cleavage by different Cas12a orthologs. The README file contains details about the content of the HTS data set, and the key to the file names and corresponding samples.</p><p><br></p> <p> </p> <p>This HTS data set supports the research article: "Pervasive off-target and double-stranded DNA nicking by CRISPR-Cas12a".</p><p><br></p><p>Version 2: New data set for pLibrary CCR5 uploaded with updated "README" file (v2).</p><p><br></p><p>Version 3: Data set corrected for pLibrary PS4 with updated "README" file (v3).</p> <p> </p> <p> </p><br></div> 2019-12-12 20:00:31 CRISPR-Cas12a/Cpf1 High-Throughput Sequencing Technology HTS CRISPR Cas12a/Cpf1 Nicking Nickase specificity Biochemistry and Cell Biology not elsewhere classified Bioinformatics Molecular Biology