High-throughput sequencing (HTS) data of plasmid library subjected to Cas12a cleavage
Datasets usually provide raw data for analysis. This raw data often comes in spreadsheet form, but can be any collection of data, on which analysis can be performed.
Cas12a (formerly Cpf1) is an RNA-guided endonuclease in the CRISPR-Cas immune system that can be easily programmed for genome editing. To understand the native cleavage specificity and cleavage activity of Cas12a, we employed high-throughput in vitro cleavage assays using a plasmid library (pLibrary). We tested the cleavage activity of three Cas12a orthologs – FnCas12a, LbCas12a and AsCas12a. For each Cas12a ortholog, we used three different crRNA sequences and corresponding target libraries (pLibrary), referred to as pLibrary PS4, EMX1 and CCR5. The data set is the high-throughput sequencing (HTS) reads of pLibrary subjected to cleavage by different Cas12a orthologs. The README file contains details about the content of the HTS data set, and the key to the file names and corresponding samples.
This HTS data set supports the research article: "Pervasive off-target and double-stranded DNA nicking by CRISPR-Cas12a".
Version 2: New data set for pLibrary CCR5 uploaded with updated "README" file (v2).
Version 3: Data set corrected for pLibrary PS4 with updated "README" file (v3).
CAREER:Defining and improving Class 2 CRISPR-Cas endonuclease sequence specificity
Directorate for Biological SciencesFind out more...