Iowa State University
327 files

High-throughput sequencing (HTS) data of plasmid library subjected to Cas12a cleavage

Version 4 2020-05-04, 20:55
Version 3 2019-12-12, 20:00
Version 2 2019-07-11, 15:05
Version 1 2019-05-28, 20:34
posted on 2020-05-04, 20:55 authored by Karthik MuruganKarthik Murugan, Dipali Sashital, Arun Seetharam, Andrew SeverinAndrew Severin

Cas12a (formerly Cpf1) is an RNA-guided endonuclease in the CRISPR-Cas immune system that can be easily programmed for genome editing. To understand the native cleavage specificity and cleavage activity of Cas12a, we employed high-throughput in vitro cleavage assays using a plasmid library (pLibrary). We tested the cleavage activity of three Cas12a orthologs – FnCas12a, LbCas12a and AsCas12a. For each Cas12a ortholog, we used three different crRNA sequences and corresponding target libraries (pLibrary), referred to as pLibrary PS4, EMX1 and CCR5. The data set is the high-throughput sequencing (HTS) reads of pLibrary subjected to cleavage by different Cas12a orthologs. The README file contains details about the content of the HTS data set, and the key to the file names and corresponding samples.

This HTS data set supports the research article: "CRISPR-Cas12a has widespread off-target and dsDNA-nicking effects".
Previous title: "Pervasive off-target and double-stranded DNA nicking by CRISPR-Cas12a".

Version 2: New data set for pLibrary CCR5 uploaded with updated "README" file (v2).

Version 3: Data set corrected for pLibrary PS4 with updated "README" file (v3).

Version 4: "README" file (v4) and reference updated with new title and link to publication.


CAREER:Defining and improving Class 2 CRISPR-Cas endonuclease sequence specificity

Directorate for Biological Sciences

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