Iowa State University
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DOCUMENT
Readme.pdf (137.25 kB)
TEXT
Plasmid sequences.txt (215.6 kB)
DATASET
UTR mut.xlsx (17.65 kB)
DATASET
UTR and LM RG under TC.xlsx (9.25 kB)
DOCUMENT
S1 wobble block rep.pdf (901.63 kB)
DATASET
S1 wobble block rep.xlsx (20.52 kB)
DOCUMENT
UTR and LM RG under TC western blot.pdf (627.43 kB)
DOCUMENT
UTR mut.pdf (470.79 kB)
DATASET
Panhandle mutants.xlsx (11.83 kB)
DOCUMENT
Panhandle mutants immunoblot.pdf (597.91 kB)
DOCUMENT
LM Immunoblot.pdf (523.23 kB)
DATASET
LM RG Immunoblot and RT-qPCR data.xlsx (15.55 kB)
DATASET
UTR and 200s all S seg.xlsx (10.08 kB)
DATASET
S1 NanoLuc.xlsx (12.16 kB)
DATASET
200s vs UTR mapping.xlsx (24.02 kB)
DOCUMENT
200s vs UTR mapping set A.pdf (371.12 MB)
DOCUMENT
200s vs UTR mapping set B.pdf (703.61 kB)
DOCUMENT
200s vs UTR mapping set C.pdf (591.4 kB)
DATASET
WB with all proteins expressed.xlsx (12.17 kB)
DATASET
Repn assay of 200-S1-200,18-S1-40 and S1WT.xlsx (10.03 kB)
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24 files

Mapping of S gene segments of Mammalian orthoreovirus.

Version 2 2024-03-06, 16:19
Version 1 2024-03-06, 15:58
dataset
posted on 2024-03-06, 16:19 authored by Debarpan DharDebarpan Dhar, Cathy MillerCathy Miller, Walter Moss, Samir Mehanovic

Determination of sequences at gene segment termini that play a critical role in Mammalian orthoreovirus S gene segment packaging. This contains the raw data from reverse genetics, plaque assays, immunoblotting, trans-complementation assay, RT-qPCR and NanoLuc assays. Data is presented in the form of .xlsx, .pdf, .tif, and .txt files.

Change log: Additional files uploaded and readme document updated 02/12/24. Additions include plasmid sequences.txt, immunofluorescence assay images 1.tif and 2.tif, immunpblot sig 1 and sNS with 9 other proteins.pdf, and three .xlsx files (WB with all proteins expressed, Repn assay of 200-S1 18-S1-40 and S1WT, and IFA of 200-S1-200 18-S1-40 and S1WT).

Related material entries added 03/06/24.

Funding

Seed Grant, College of Veterinary Medicine, Iowa State University

National Institute of Health-National Cancer Institute Grant

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