Supplemental data for "A fat- and sucrose-enriched diet causes metabolic alterations in mdx mice."

Duchenne muscular dystrophy (DMD) is a fatal muscle disease that affects 1 in 5000 boys born worldwide. Metabolic complications such as obesity and/or insulin resistance is often associated with DMD. However, the effect of obesity and/or insulin resistance on DMD is not well understood. As part of the experiments to understand the impact of a high fat, high sucrose diet (HFHSD) on DMD, we performed lipidomics (using serum) and metabolomics (using quadriceps muscles). The datasets here are the supplemental data for the research article "A fat- and sucrose-enriched diet causes metabolic alterations in mdx mice" published in The American Journal of Physiology-Regulatory, Integrative and Comparative Physiology.

Files contained in the data set include:

• Supplementary figure. 1. Volcano plots demonstrating differential expression of lipid species with log2 fold changes (log2FC) on the x-axis and log10 non-adjusted p-values (log10 p value) on the y-axis for the various comparisons. Volcano plots showing features that are increased and decreased in 1a) C57 HFHSD when compared to C57 CD 1b) mdx HFHSD when compared to mdx CD 1c) mdx CD when compared to C57 CD 1d) mdx HFHSD when compared to C57 HFHSD. Differential expression was determined by the q-value =0.1 and log2FC =0.2.
• Supplementary figure 2. 2a) A heat map with significant features from detected metabolites analyzed using ANOVA2 (FDR=0.25), clustered by the Euclidean-Ward method. 2b) sPLS-DA plot explaining the variability of the features in the 4 groups based on the first two components performed for the metabolomics dataset. 2c) Pathway enrichment analysis performed on all the significant features from ANOVA2 for the detected metabolites. 2d) Location enrichment for organ, tissue, and subcellular localization performed on all the significant features from metabolomics following ANOVA2 analyses.
• Supplementary table. 1 Common and unique lipid species identified in the Venn diagrams created using the comparisons of interest.
• MetabolomicsLipidomicsDE.xlsx contains differential expression from both lipidomics and metabolomics data analyses.