The data set is the high-throughput sequencing (HTS) reads of samples generated in the paper titled "High-throughput in vitro specificity profiling of natural and high-fidelity CRISPR-Cas9 variants". This README files contains details about the contents of the HTS data set. IMPORTANT: Please refer to the METHODS section of the paper for further details on bioinformatic analysis. ************************************************** Notes on fastq files: > all HTS files are in compressed fastq format > see "Sample key" below for information regarding sample type (pLibrary, Cas9, time points) > N = nicked SC = supercoiled > Sp = SpCas9 Sa = SaCas9 HF1 = SpCas9 HF1 Hypa = HypaCas9 HiFi or IDT = IDT Alt-R HiFi Cas9 > 002 = pLibrary PS4 = KMlib002 003 = pLibrary EMX1 = KMlib003 > Trials (1 and 2) are denoted as [rep1, rep2], or [rep4R] > Samples have two fastq files each, R1 and R2 reads. These are 75x PE reads and have to be joined to get target region ========== Sample key for time points: Numbers 2-11 indicate supercoiled or nicked fractions at various time points shown below: time supercoiled nicked 0 SC N - 0h control (pLibrary run on a gel after preparation) 1 min 2 3 - experimental sample 5 min 4 5 - experimental sample 30 min 6 7 - experimental sample 1 h 8 9 - experimental sample 3 h 10 11 - experimental sample ========== Sample key for files: [S. No] [FASTQ FILE NAME] [SAMPLE NAME] [NOTES] 1 1786FL-12-01-373_S373 002_SC_rep1 Control 0 for KMlib002 - SC fraction - trial 1 2 1786FL-12-01-374_S374 002_SC_rep2 Control 0 for KMlib002 - SC fraction - trial 2 3 1786FL-12-01-376_S376 002_N_rep1 Control 0 for KMlib002 - N fraction - trial 1 4 1786FL-12-01-377_S377 002_N_rep2 Control 0 for KMlib002 - N fraction - trial 2 5 1786FL-12-01-379_S379 003_SC_rep1 Control 0 for KMlib003 - SC fraction - trial 1 6 1786FL-12-01-380_S380 003_SC_rep2 Control 0 for KMlib003 - SC fraction - trial 2 7 1786FL-12-01-382_S382 003_N_rep1 Control 0 for KMlib003 - N fraction - trial 1 8 1786FL-12-01-383_S383 003_N_rep2 Control 0 for KMlib003 - N fraction - trial 2 9 1786FL-12-01-02_S2 Sp_002_rep1_2 SpCas9 - KMlib002 - trial 1 - time point 2 10 1786FL-12-01-03_S3 Sp_002_rep1_3 SpCas9 - KMlib002 - trial 1 - time point 3 11 1786FL-12-01-04_S4 Sp_002_rep1_4 SpCas9 - KMlib002 - trial 1 - time point 4 12 1786FL-12-01-05_S5 Sp_002_rep1_5 SpCas9 - KMlib002 - trial 1 - time point 5 13 1786FL-12-01-06_S6 Sp_002_rep1_6 SpCas9 - KMlib002 - trial 1 - time point 6 14 1786FL-12-01-07_S7 Sp_002_rep1_7 SpCas9 - KMlib002 - trial 1 - time point 7 15 1786FL-12-01-08_S8 Sp_002_rep1_8 SpCas9 - KMlib002 - trial 1 - time point 8 16 1786FL-12-01-09_S9 Sp_002_rep1_9 SpCas9 - KMlib002 - trial 1 - time point 9 17 1786FL-12-01-10_S10 Sp_002_rep1_10 SpCas9 - KMlib002 - trial 1 - time point 10 18 1786FL-12-01-11_S11 Sp_002_rep1_11 SpCas9 - KMlib002 - trial 1 - time point 11 19 Sp-002-rep4R-2_S1 Sp_002_rep4R_2 SpCas9 - KMlib002 - trial 2 - time point 2 20 Sp-002-rep4R-3_S2 Sp_002_rep4R_3 SpCas9 - KMlib002 - trial 2 - time point 3 21 Sp-002-rep4R-4_S3 Sp_002_rep4R_4 SpCas9 - KMlib002 - trial 2 - time point 4 22 Sp-002-rep4R-5_S4 Sp_002_rep4R_5 SpCas9 - KMlib002 - trial 2 - time point 5 23 Sp-002-rep4R-6_S5 Sp_002_rep4R_6 SpCas9 - KMlib002 - trial 2 - time point 6 24 Sp-002-rep4R-7_S6 Sp_002_rep4R_7 SpCas9 - KMlib002 - trial 2 - time point 7 25 Sp-002-rep4R-8_S7 Sp_002_rep4R_8 SpCas9 - KMlib002 - trial 2 - time point 8 26 Sp-002-rep4R-9_S8 Sp_002_rep4R_9 SpCas9 - KMlib002 - trial 2 - time point 9 27 Sp-002-rep4R-10_S9 Sp_002_rep4R_10 SpCas9 - KMlib002 - trial 2 - time point 10 28 Sp-002-rep4R-11_S10 Sp_002_rep4R_11 SpCas9 - KMlib002 - trial 2 - time point 11 29 1786FL-12-01-26_S26 Sp_003_rep1_2 SpCas9 - KMlib003 - trial 1 - time point 2 30 1786FL-12-01-27_S27 Sp_003_rep1_3 SpCas9 - KMlib003 - trial 1 - time point 3 31 1786FL-12-01-28_S28 Sp_003_rep1_4 SpCas9 - KMlib003 - trial 1 - time point 4 32 1786FL-12-01-29_S29 Sp_003_rep1_5 SpCas9 - KMlib003 - trial 1 - time point 5 33 1786FL-12-01-30_S30 Sp_003_rep1_6 SpCas9 - KMlib003 - trial 1 - time point 6 34 1786FL-12-01-31_S31 Sp_003_rep1_7 SpCas9 - KMlib003 - trial 1 - time point 7 35 1786FL-12-01-32_S32 Sp_003_rep1_8 SpCas9 - KMlib003 - trial 1 - time point 8 36 1786FL-12-01-33_S33 Sp_003_rep1_9 SpCas9 - KMlib003 - trial 1 - time point 9 37 1786FL-12-01-34_S34 Sp_003_rep1_10 SpCas9 - KMlib003 - trial 1 - time point 10 38 1786FL-12-01-35_S35 Sp_003_rep1_11 SpCas9 - KMlib003 - trial 1 - time point 11 39 1786FL-12-01-38_S38 Sp_003_rep2_2 SpCas9 - KMlib003 - trial 2 - time point 2 40 1786FL-12-01-39_S39 Sp_003_rep2_3 SpCas9 - KMlib003 - trial 2 - time point 3 41 1786FL-12-01-40_S40 Sp_003_rep2_4 SpCas9 - KMlib003 - trial 2 - time point 4 42 1786FL-12-01-41_S41 Sp_003_rep2_5 SpCas9 - KMlib003 - trial 2 - time point 5 43 1786FL-12-01-42_S42 Sp_003_rep2_6 SpCas9 - KMlib003 - trial 2 - time point 6 44 1786FL-12-01-43_S43 Sp_003_rep2_7 SpCas9 - KMlib003 - trial 2 - time point 7 45 1786FL-12-01-44_S44 Sp_003_rep2_8 SpCas9 - KMlib003 - trial 2 - time point 8 46 1786FL-12-01-45_S45 Sp_003_rep2_9 SpCas9 - KMlib003 - trial 2 - time point 9 47 1786FL-12-01-46_S46 Sp_003_rep2_10 SpCas9 - KMlib003 - trial 2 - time point 10 48 1786FL-12-01-47_S47 Sp_003_rep2_11 SpCas9 - KMlib003 - trial 2 - time point 11 49 1786FL-12-01-86_S86 Sa_002_rep2_2 SaCas9 - KMlib002 - trial 1 - time point 2 50 1786FL-12-01-87_S87 Sa_002_rep2_3 SaCas9 - KMlib002 - trial 1 - time point 3 51 1786FL-12-01-88_S88 Sa_002_rep2_4 SaCas9 - KMlib002 - trial 1 - time point 4 52 1786FL-12-01-89_S89 Sa_002_rep2_5 SaCas9 - KMlib002 - trial 1 - time point 5 53 1786FL-12-01-90_S90 Sa_002_rep2_6 SaCas9 - KMlib002 - trial 1 - time point 6 54 1786FL-12-01-91_S91 Sa_002_rep2_7 SaCas9 - KMlib002 - trial 1 - time point 7 55 1786FL-12-01-92_S92 Sa_002_rep2_8 SaCas9 - KMlib002 - trial 1 - time point 8 56 1786FL-12-01-93_S93 Sa_002_rep2_9 SaCas9 - KMlib002 - trial 1 - time point 9 57 1786FL-12-01-94_S94 Sa_002_rep2_10 SaCas9 - KMlib002 - trial 1 - time point 10 58 1786FL-12-01-95_S95 Sa_002_rep2_11 SaCas9 - KMlib002 - trial 1 - time point 11 59 Sa-002-rep4R-2_S21 Sa_002_rep4R_2 SaCas9 - KMlib002 - trial 2 - time point 2 60 Sa-002-rep4R-3_S22 Sa_002_rep4R_3 SaCas9 - KMlib002 - trial 2 - time point 3 61 Sa-002-rep4R-4_S23 Sa_002_rep4R_4 SaCas9 - KMlib002 - trial 2 - time point 4 62 Sa-002-rep4R-5_S24 Sa_002_rep4R_5 SaCas9 - KMlib002 - trial 2 - time point 5 63 Sa-002-rep4R-6_S25 Sa_002_rep4R_6 SaCas9 - KMlib002 - trial 2 - time point 6 64 Sa-002-rep4R-7_S26 Sa_002_rep4R_7 SaCas9 - KMlib002 - trial 2 - time point 7 65 Sa-002-rep4R-8_S27 Sa_002_rep4R_8 SaCas9 - KMlib002 - trial 2 - time point 8 66 Sa-002-rep4R-9_S28 Sa_002_rep4R_9 SaCas9 - KMlib002 - trial 2 - time point 9 67 Sa-002-rep4R-10_S29 Sa_002_rep4R_10 SaCas9 - KMlib002 - trial 2 - time point 10 68 Sa-002-rep4R-11_S30 Sa_002_rep4R_11 SaCas9 - KMlib002 - trial 2 - time point 11 69 1786FL-12-01-98_S98 Sa_003_rep1_2 SaCas9 - KMlib003 - trial 1 - time point 2 70 1786FL-12-01-99_S99 Sa_003_rep1_3 SaCas9 - KMlib003 - trial 1 - time point 3 71 1786FL-12-01-100_S100 Sa_003_rep1_4 SaCas9 - KMlib003 - trial 1 - time point 4 72 1786FL-12-01-101_S101 Sa_003_rep1_5 SaCas9 - KMlib003 - trial 1 - time point 5 73 1786FL-12-01-102_S102 Sa_003_rep1_6 SaCas9 - KMlib003 - trial 1 - time point 6 74 1786FL-12-01-103_S103 Sa_003_rep1_7 SaCas9 - KMlib003 - trial 1 - time point 7 75 1786FL-12-01-104_S104 Sa_003_rep1_8 SaCas9 - KMlib003 - trial 1 - time point 8 76 1786FL-12-01-105_S105 Sa_003_rep1_9 SaCas9 - KMlib003 - trial 1 - time point 9 77 1786FL-12-01-106_S106 Sa_003_rep1_10 SaCas9 - KMlib003 - trial 1 - time point 10 78 1786FL-12-01-107_S107 Sa_003_rep1_11 SaCas9 - KMlib003 - trial 1 - time point 11 79 1786FL-12-01-110_S110 Sa_003_rep2_2 SaCas9 - KMlib003 - trial 2 - time point 2 80 1786FL-12-01-111_S111 Sa_003_rep2_3 SaCas9 - KMlib003 - trial 2 - time point 3 81 1786FL-12-01-112_S112 Sa_003_rep2_4 SaCas9 - KMlib003 - trial 2 - time point 4 82 1786FL-12-01-113_S113 Sa_003_rep2_5 SaCas9 - KMlib003 - trial 2 - time point 5 83 1786FL-12-01-114_S114 Sa_003_rep2_6 SaCas9 - KMlib003 - trial 2 - time point 6 84 1786FL-12-01-115_S115 Sa_003_rep2_7 SaCas9 - KMlib003 - trial 2 - time point 7 85 1786FL-12-01-116_S116 Sa_003_rep2_8 SaCas9 - KMlib003 - trial 2 - time point 8 86 1786FL-12-01-117_S117 Sa_003_rep2_9 SaCas9 - KMlib003 - trial 2 - time point 9 87 1786FL-12-01-118_S118 Sa_003_rep2_10 SaCas9 - KMlib003 - trial 2 - time point 10 88 1786FL-12-01-119_S119 Sa_003_rep2_11 SaCas9 - KMlib003 - trial 2 - time point 11 89 1786FL-12-01-146_S146 IDT_002_rep1_2 HiFi Cas9 - KMlib002 - trial 1 - time point 2 90 1786FL-12-01-147_S147 IDT_002_rep1_3 HiFi Cas9 - KMlib002 - trial 1 - time point 3 91 1786FL-12-01-148_S148 IDT_002_rep1_4 HiFi Cas9 - KMlib002 - trial 1 - time point 4 92 1786FL-12-01-149_S149 IDT_002_rep1_5 HiFi Cas9 - KMlib002 - trial 1 - time point 5 93 1786FL-12-01-150_S150 IDT_002_rep1_6 HiFi Cas9 - KMlib002 - trial 1 - time point 6 94 1786FL-12-01-151_S151 IDT_002_rep1_7 HiFi Cas9 - KMlib002 - trial 1 - time point 7 95 1786FL-12-01-152_S152 IDT_002_rep1_8 HiFi Cas9 - KMlib002 - trial 1 - time point 8 96 1786FL-12-01-153_S153 IDT_002_rep1_9 HiFi Cas9 - KMlib002 - trial 1 - time point 9 97 1786FL-12-01-154_S154 IDT_002_rep1_10 HiFi Cas9 - KMlib002 - trial 1 - time point 10 98 1786FL-12-01-155_S155 IDT_002_rep1_11 HiFi Cas9 - KMlib002 - trial 1 - time point 11 99 1786FL-12-01-158_S158 IDT_002_rep2_2 HiFi Cas9 - KMlib002 - trial 2 - time point 2 100 1786FL-12-01-159_S159 IDT_002_rep2_3 HiFi Cas9 - KMlib002 - trial 2 - time point 3 101 1786FL-12-01-160_S160 IDT_002_rep2_4 HiFi Cas9 - KMlib002 - trial 2 - time point 4 102 1786FL-12-01-161_S161 IDT_002_rep2_5 HiFi Cas9 - KMlib002 - trial 2 - time point 5 103 1786FL-12-01-162_S162 IDT_002_rep2_6 HiFi Cas9 - KMlib002 - trial 2 - time point 6 104 1786FL-12-01-163_S163 IDT_002_rep2_7 HiFi Cas9 - KMlib002 - trial 2 - time point 7 105 1786FL-12-01-164_S164 IDT_002_rep2_8 HiFi Cas9 - KMlib002 - trial 2 - time point 8 106 1786FL-12-01-165_S165 IDT_002_rep2_9 HiFi Cas9 - KMlib002 - trial 2 - time point 9 107 1786FL-12-01-166_S166 IDT_002_rep2_10 HiFi Cas9 - KMlib002 - trial 2 - time point 10 108 1786FL-12-01-167_S167 IDT_002_rep2_11 HiFi Cas9 - KMlib002 - trial 2 - time point 11 109 1786FL-12-01-170_S170 IDT_003_rep1_2 HiFi Cas9 - KMlib003 - trial 1 - time point 2 110 1786FL-12-01-171_S171 IDT_003_rep1_3 HiFi Cas9 - KMlib003 - trial 1 - time point 3 111 1786FL-12-01-172_S172 IDT_003_rep1_4 HiFi Cas9 - KMlib003 - trial 1 - time point 4 112 1786FL-12-01-173_S173 IDT_003_rep1_5 HiFi Cas9 - KMlib003 - trial 1 - time point 5 113 1786FL-12-01-174_S174 IDT_003_rep1_6 HiFi Cas9 - KMlib003 - trial 1 - time point 6 114 1786FL-12-01-175_S175 IDT_003_rep1_7 HiFi Cas9 - KMlib003 - trial 1 - time point 7 115 1786FL-12-01-176_S176 IDT_003_rep1_8 HiFi Cas9 - KMlib003 - trial 1 - time point 8 116 1786FL-12-01-177_S177 IDT_003_rep1_9 HiFi Cas9 - KMlib003 - trial 1 - time point 9 117 1786FL-12-01-178_S178 IDT_003_rep1_10 HiFi Cas9 - KMlib003 - trial 1 - time point 10 118 1786FL-12-01-179_S179 IDT_003_rep1_11 HiFi Cas9 - KMlib003 - trial 1 - time point 11 119 1786FL-12-01-182_S182 IDT_003_rep2_2 HiFi Cas9 - KMlib003 - trial 2 - time point 2 120 1786FL-12-01-183_S183 IDT_003_rep2_3 HiFi Cas9 - KMlib003 - trial 2 - time point 3 121 1786FL-12-01-184_S184 IDT_003_rep2_4 HiFi Cas9 - KMlib003 - trial 2 - time point 4 122 1786FL-12-01-185_S185 IDT_003_rep2_5 HiFi Cas9 - KMlib003 - trial 2 - time point 5 123 1786FL-12-01-186_S186 IDT_003_rep2_6 HiFi Cas9 - KMlib003 - trial 2 - time point 6 124 1786FL-12-01-187_S187 IDT_003_rep2_7 HiFi Cas9 - KMlib003 - trial 2 - time point 7 125 1786FL-12-01-188_S188 IDT_003_rep2_8 HiFi Cas9 - KMlib003 - trial 2 - time point 8 126 1786FL-12-01-189_S189 IDT_003_rep2_9 HiFi Cas9 - KMlib003 - trial 2 - time point 9 127 1786FL-12-01-190_S190 IDT_003_rep2_10 HiFi Cas9 - KMlib003 - trial 2 - time point 10 128 1786FL-12-01-191_S191 IDT_003_rep2_11 HiFi Cas9 - KMlib003 - trial 2 - time point 11 129 1786FL-12-01-218_S218 Hypa_002_rep1_2 Hypa Cas9 - KMlib002 - trial 1 - time point 2 130 1786FL-12-01-219_S219 Hypa_002_rep1_3 Hypa Cas9 - KMlib002 - trial 1 - time point 3 131 1786FL-12-01-220_S220 Hypa_002_rep1_4 Hypa Cas9 - KMlib002 - trial 1 - time point 4 132 1786FL-12-01-221_S221 Hypa_002_rep1_5 Hypa Cas9 - KMlib002 - trial 1 - time point 5 133 1786FL-12-01-222_S222 Hypa_002_rep1_6 Hypa Cas9 - KMlib002 - trial 1 - time point 6 134 1786FL-12-01-223_S223 Hypa_002_rep1_7 Hypa Cas9 - KMlib002 - trial 1 - time point 7 135 1786FL-12-01-224_S224 Hypa_002_rep1_8 Hypa Cas9 - KMlib002 - trial 1 - time point 8 136 1786FL-12-01-225_S225 Hypa_002_rep1_9 Hypa Cas9 - KMlib002 - trial 1 - time point 9 137 1786FL-12-01-226_S226 Hypa_002_rep1_10 Hypa Cas9 - KMlib002 - trial 1 - time point 10 138 1786FL-12-01-227_S227 Hypa_002_rep1_11 Hypa Cas9 - KMlib002 - trial 1 - time point 11 139 1786FL-12-01-230_S230 Hypa_002_rep2_2 Hypa Cas9 - KMlib002 - trial 2 - time point 2 140 1786FL-12-01-231_S231 Hypa_002_rep2_3 Hypa Cas9 - KMlib002 - trial 2 - time point 3 141 1786FL-12-01-232_S232 Hypa_002_rep2_4 Hypa Cas9 - KMlib002 - trial 2 - time point 4 142 1786FL-12-01-233_S233 Hypa_002_rep2_5 Hypa Cas9 - KMlib002 - trial 2 - time point 5 143 1786FL-12-01-234_S234 Hypa_002_rep2_6 Hypa Cas9 - KMlib002 - trial 2 - time point 6 144 1786FL-12-01-235_S235 Hypa_002_rep2_7 Hypa Cas9 - KMlib002 - trial 2 - time point 7 145 1786FL-12-01-236_S236 Hypa_002_rep2_8 Hypa Cas9 - KMlib002 - trial 2 - time point 8 146 1786FL-12-01-237_S237 Hypa_002_rep2_9 Hypa Cas9 - KMlib002 - trial 2 - time point 9 147 1786FL-12-01-238_S238 Hypa_002_rep2_10 Hypa Cas9 - KMlib002 - trial 2 - time point 10 148 1786FL-12-01-239_S239 Hypa_002_rep2_11 Hypa Cas9 - KMlib002 - trial 2 - time point 11 149 1786FL-12-01-242_S242 Hypa_003_rep1_2 Hypa Cas9 - KMlib003 - trial 1 - time point 2 150 1786FL-12-01-243_S243 Hypa_003_rep1_3 Hypa Cas9 - KMlib003 - trial 1 - time point 3 151 1786FL-12-01-244_S244 Hypa_003_rep1_4 Hypa Cas9 - KMlib003 - trial 1 - time point 4 152 1786FL-12-01-245_S245 Hypa_003_rep1_5 Hypa Cas9 - KMlib003 - trial 1 - time point 5 153 1786FL-12-01-246_S246 Hypa_003_rep1_6 Hypa Cas9 - KMlib003 - trial 1 - time point 6 154 1786FL-12-01-247_S247 Hypa_003_rep1_7 Hypa Cas9 - KMlib003 - trial 1 - time point 7 155 1786FL-12-01-248_S248 Hypa_003_rep1_8 Hypa Cas9 - KMlib003 - trial 1 - time point 8 156 1786FL-12-01-249_S249 Hypa_003_rep1_9 Hypa Cas9 - KMlib003 - trial 1 - time point 9 157 1786FL-12-01-250_S250 Hypa_003_rep1_10 Hypa Cas9 - KMlib003 - trial 1 - time point 10 158 1786FL-12-01-251_S251 Hypa_003_rep1_11 Hypa Cas9 - KMlib003 - trial 1 - time point 11 159 1786FL-12-01-254_S254 Hypa_003_rep2_2 Hypa Cas9 - KMlib003 - trial 2 - time point 2 160 1786FL-12-01-255_S255 Hypa_003_rep2_3 Hypa Cas9 - KMlib003 - trial 2 - time point 3 161 1786FL-12-01-256_S256 Hypa_003_rep2_4 Hypa Cas9 - KMlib003 - trial 2 - time point 4 162 1786FL-12-01-257_S257 Hypa_003_rep2_5 Hypa Cas9 - KMlib003 - trial 2 - time point 5 163 1786FL-12-01-258_S258 Hypa_003_rep2_6 Hypa Cas9 - KMlib003 - trial 2 - time point 6 164 1786FL-12-01-259_S259 Hypa_003_rep2_7 Hypa Cas9 - KMlib003 - trial 2 - time point 7 165 1786FL-12-01-260_S260 Hypa_003_rep2_8 Hypa Cas9 - KMlib003 - trial 2 - time point 8 166 1786FL-12-01-261_S261 Hypa_003_rep2_9 Hypa Cas9 - KMlib003 - trial 2 - time point 9 167 1786FL-12-01-262_S262 Hypa_003_rep2_10 Hypa Cas9 - KMlib003 - trial 2 - time point 10 168 1786FL-12-01-263_S263 Hypa_003_rep2_11 Hypa Cas9 - KMlib003 - trial 2 - time point 11 169 1786FL-12-01-290_S290 HF1_002_rep1_2 SpCas9 HF1 - KMlib002 - trial 1 - time point 2 170 1786FL-12-01-291_S291 HF1_002_rep1_3 SpCas9 HF1 - KMlib002 - trial 1 - time point 3 171 1786FL-12-01-292_S292 HF1_002_rep1_4 SpCas9 HF1 - KMlib002 - trial 1 - time point 4 172 1786FL-12-01-293_S293 HF1_002_rep1_5 SpCas9 HF1 - KMlib002 - trial 1 - time point 5 173 1786FL-12-01-294_S294 HF1_002_rep1_6 SpCas9 HF1 - KMlib002 - trial 1 - time point 6 174 1786FL-12-01-295_S295 HF1_002_rep1_7 SpCas9 HF1 - KMlib002 - trial 1 - time point 7 175 1786FL-12-01-296_S296 HF1_002_rep1_8 SpCas9 HF1 - KMlib002 - trial 1 - time point 8 176 1786FL-12-01-297_S297 HF1_002_rep1_9 SpCas9 HF1 - KMlib002 - trial 1 - time point 9 177 1786FL-12-01-298_S298 HF1_002_rep1_10 SpCas9 HF1 - KMlib002 - trial 1 - time point 10 178 1786FL-12-01-299_S299 HF1_002_rep1_11 SpCas9 HF1 - KMlib002 - trial 1 - time point 11 179 1786FL-12-01-302_S302 HF1_002_rep2_2 SpCas9 HF1 - KMlib002 - trial 2 - time point 2 180 1786FL-12-01-303_S303 HF1_002_rep2_3 SpCas9 HF1 - KMlib002 - trial 2 - time point 3 181 1786FL-12-01-304_S304 HF1_002_rep2_4 SpCas9 HF1 - KMlib002 - trial 2 - time point 4 182 1786FL-12-01-305_S305 HF1_002_rep2_5 SpCas9 HF1 - KMlib002 - trial 2 - time point 5 183 1786FL-12-01-306_S306 HF1_002_rep2_6 SpCas9 HF1 - KMlib002 - trial 2 - time point 6 184 1786FL-12-01-307_S307 HF1_002_rep2_7 SpCas9 HF1 - KMlib002 - trial 2 - time point 7 185 1786FL-12-01-308_S308 HF1_002_rep2_8 SpCas9 HF1 - KMlib002 - trial 2 - time point 8 186 1786FL-12-01-309_S309 HF1_002_rep2_9 SpCas9 HF1 - KMlib002 - trial 2 - time point 9 187 1786FL-12-01-310_S310 HF1_002_rep2_10 SpCas9 HF1 - KMlib002 - trial 2 - time point 10 188 1786FL-12-01-311_S311 HF1_002_rep2_11 SpCas9 HF1 - KMlib002 - trial 2 - time point 11 189 1786FL-12-01-314_S314 HF1_003_rep1_2 SpCas9 HF1 - KMlib003 - trial 1 - time point 2 190 1786FL-12-01-315_S315 HF1_003_rep1_3 SpCas9 HF1 - KMlib003 - trial 1 - time point 3 191 1786FL-12-01-316_S316 HF1_003_rep1_4 SpCas9 HF1 - KMlib003 - trial 1 - time point 4 192 1786FL-12-01-317_S317 HF1_003_rep1_5 SpCas9 HF1 - KMlib003 - trial 1 - time point 5 193 1786FL-12-01-318_S318 HF1_003_rep1_6 SpCas9 HF1 - KMlib003 - trial 1 - time point 6 194 1786FL-12-01-319_S319 HF1_003_rep1_7 SpCas9 HF1 - KMlib003 - trial 1 - time point 7 195 1786FL-12-01-320_S320 HF1_003_rep1_8 SpCas9 HF1 - KMlib003 - trial 1 - time point 8 196 1786FL-12-01-321_S321 HF1_003_rep1_9 SpCas9 HF1 - KMlib003 - trial 1 - time point 9 197 1786FL-12-01-322_S322 HF1_003_rep1_10 SpCas9 HF1 - KMlib003 - trial 1 - time point 10 198 1786FL-12-01-323_S323 HF1_003_rep1_11 SpCas9 HF1 - KMlib003 - trial 1 - time point 11 199 1786FL-12-01-326_S326 HF1_003_rep2_2 SpCas9 HF1 - KMlib003 - trial 2 - time point 2 200 1786FL-12-01-327_S327 HF1_003_rep2_3 SpCas9 HF1 - KMlib003 - trial 2 - time point 3 201 1786FL-12-01-328_S328 HF1_003_rep2_4 SpCas9 HF1 - KMlib003 - trial 2 - time point 4 202 1786FL-12-01-329_S329 HF1_003_rep2_5 SpCas9 HF1 - KMlib003 - trial 2 - time point 5 203 1786FL-12-01-330_S330 HF1_003_rep2_6 SpCas9 HF1 - KMlib003 - trial 2 - time point 6 204 1786FL-12-01-331_S331 HF1_003_rep2_7 SpCas9 HF1 - KMlib003 - trial 2 - time point 7 205 1786FL-12-01-332_S332 HF1_003_rep2_8 SpCas9 HF1 - KMlib003 - trial 2 - time point 8 206 1786FL-12-01-333_S333 HF1_003_rep2_9 SpCas9 HF1 - KMlib003 - trial 2 - time point 9 207 1786FL-12-01-334_S334 HF1_003_rep2_10 SpCas9 HF1 - KMlib003 - trial 2 - time point 10 208 1786FL-12-01-335_S335 HF1_003_rep2_11 SpCas9 HF1 - KMlib003 - trial 2 - time point 11 ************************************************** Notes on CSV files: > CVS files titled "pLibrary_<>_ExtractedCountsData_mismatches" are extracted sequences and counts information > kmer denotes the target sequence > N = nicked SC = supercoiled > Sp = SpCas9 Sa = SaCas9 HF1 = SpCas9 HF1 Hypa = HypaCas9 HiFi or IDT = IDT Alt-R HiFi Cas9 > 002 = pLibrary PS4 = KMlib002 003 = pLibrary EMX1 = KMlib003 > Trials (1 and 2) are denoted as [rep1, rep2], or [rep4R] > Numbers 2-11 indicate supercoiled or nicked fractions at various time points shown below: time supercoiled nicked 0 SC N - 0h control (pLibrary run on a gel after preparation) 1 min 2 3 - experimental sample 5 min 4 5 - experimental sample 30 min 6 7 - experimental sample 1 h 8 9 - experimental sample 3 h 10 11 - experimental sample **************************************************