Sequence determinants of Cas9 cleavage activity - nicked pool Raw data for figure 5 and supplementary figure 8, 9 > Sp = SpCas9 Sa = SaCas9 HF1 = SpCas9 HF1 Hypa = HypaCas9 HiFi or IDT = IDT Alt-R HiFi Cas9 > 002 = pLibrary PS4 = KMlib002 003 = pLibrary EMX1 = KMlib003 > MM = mismatch ************************************************** pLibrary PS4 - nicked pool ΔRA and Nmax for target sequences with different misamtches (MM) (1 to 6 MM) across the target > SpCas9 Sp - ΔRA - 1MM G G A A A T T A G G T G C G C T T G G C A -0.00134668 0.001246709 0.001314299 0.001281015 -0.006310871 -0.02086637 -0.02060425 -0.002183139 1.05864E-05 3.98469E-05 -0.002485831 -0.00021954 0.003329779 -0.001610356 0.001341762 G 0.000229923 0.001342933 -0.00268244 -0.00591264 0.0012352 0.003619066 -0.005286035 -0.008859003 -0.039321064 -0.005845856 -0.004316622 -0.004739351 0.000176853 -0.002886228 -0.000781128 0.003702876 0.000703884 C 0.003387389 -0.001697346 0.002669698 0.00203392 -0.009296437 -0.003300594 0.001981463 -0.001786248 0.002531689 -0.001296056 0.002262966 -0.001327476 T 0.000781474 0.000253037 0.001257065 -0.001244993 -0.010980192 -0.002273432 0.00015978 0.000269019 -0.002118155 -0.001598772 -0.002671097 -0.001172186 -0.005462726 -0.00131834 0.000147357 0.000274258 Sp - Nmax- 1MM G G A A A T T A G G T G C G C T T G G C A 0.290766142 0.228530183 0.302766368 0.29907479 0.495469161 0.681163688 0.678757044 0.245997258 0.301060394 0.284088569 0.366279704 0.489712195 0.565270428 0.396901342 0.45038231 G 0.255023453 0.294965861 0.419050346 0.403697782 0.458367938 0.299025556 0.359086233 0.465078935 1 0.37492045 0.3127903 0.307307433 0.350009504 0.501142099 0.455740475 0.56429784 0.411102814 C 0.299611928 0.312079207 0.341979536 0.462573328 0.586130708 0.397933597 0.363532548 0.46700058 0.797450531 0.432077727 0.566933322 0.61077033 T 0.30596658 0.356330854 0.509896983 0.386938519 0.487161743 0.295906781 0.242271388 0.330223554 0.357533437 0.294617008 0.468190242 0.28042825 0.424831445 0.285209774 0.302017394 0.56308439 Sp - ΔRA - 2MM G G A A A T T A G G T G C G C T T G G C A -0.003467089 -0.001079165 -0.005123024 -0.001199544 -0.005576587 -0.008693925 -0.006348439 -0.00449037 -0.003817384 -0.001013005 -0.001697025 0.001132986 0.015422737 0.000657371 0.000802169 G -0.004736472 -0.000422079 0.002139831 -0.002397957 -0.002454766 -0.00333073 -0.012385756 -0.004520019 -0.010769414 -0.008454434 -0.003475635 -0.003918477 -0.002742947 -0.000175023 -0.003687427 0.009902228 0.001131005 C -0.000605414 -0.00099572 -0.003070027 -0.002525916 -0.005687798 -0.00442156 -0.004079114 -0.002307747 -0.000968141 -0.000943488 0.012895283 0.002000761 T -0.001123834 -0.002361219 -0.010204276 -0.005106256 -0.019461795 -0.011536178 -0.004727481 -0.008181841 -0.001542026 -0.006350519 -0.002039137 -0.003749474 0.001322868 0.000769557 0.001359397 -0.000723631 Sp - Nmax- 2MM G G A A A T T A G G T G C G C T T G G C A 0.344891138 0.298693213 0.403096131 0.337592259 0.665160158 0.772511351 0.757851823 0.439725836 0.471678973 0.352634274 0.449741032 0.53417327 0.911164674 0.43713144 0.540701673 G 0.413699748 0.400820055 0.573940816 0.471358716 0.643958201 0.330146613 0.644560038 0.527220226 1 0.493539277 0.385238022 0.383549012 0.395988513 0.483506583 0.521584366 0.61815347 0.538203989 C 0.367250917 0.331719925 0.494158632 0.488754813 0.643812015 0.465802044 0.464791057 0.537190839 0.914909881 0.532280034 0.998500829 0.793357129 T 0.274390188 0.396650074 0.723257158 0.535964484 0.787561958 0.566476991 0.395240053 0.510440032 0.50966649 0.458355742 0.520658686 0.435201875 0.49228831 0.435390009 0.42379114 0.827981024 Sp - ΔRA - 3MM G G A A A T T A G G T G C G C T T G G C A -0.000190894 0.003736259 0.000391076 0.001094723 0.009527406 0.00879099 0.009939661 0.002336569 0.003513205 0.000134041 -0.000461966 0.000876673 0.011700556 0.004503113 0.002180691 G 0.003290822 0.007157262 0.014377462 0.008032096 0.007946899 0.000617263 -0.002075371 0.006755197 0.011405138 -0.004508806 -0.000460904 0.002227508 -8.62052E-05 -0.000550455 -0.002560185 0.012076254 0.002555505 C 0.00458912 0.002561413 0.004343963 0.005736222 0.007066135 0.00130254 0.000999003 0.004535544 0.003098893 0.002497962 0.005113618 0.002198207 T -0.002291859 0.006375217 0.002320041 0.003466681 0.000478975 -0.000263209 0.001261605 -0.003134543 0.001562603 -0.00139731 0.001527545 0.003151976 0.008493479 0.008025925 0.003402769 0.003728058 Sp - Nmax- 3MM G G A A A T T A G G T G C G C T T G G C A 0.417534444 0.438902944 0.460441797 0.422318091 0.778262259 0.849714576 0.830734739 0.497696637 0.550335771 0.376127847 0.419959887 0.503867772 0.706878382 0.485135837 0.534723242 G 0.533768108 0.61096854 0.900541901 0.655036346 0.784563242 0.3413737 0.589810135 0.718123353 1 0.483636585 0.421821161 0.462190337 0.424909802 0.490808415 0.461649602 0.722845484 0.488068971 C 0.499951946 0.432492783 0.56196043 0.570504334 0.719248681 0.525660953 0.526428004 0.662787204 0.597944395 0.504380932 0.642757595 0.658656286 T 0.313992982 0.540956547 0.635414923 0.514032263 0.625917238 0.451121607 0.386974846 0.465585743 0.568260455 0.436925198 0.491985858 0.476085823 0.621015119 0.428423134 0.479012673 0.641551648 Sp - ΔRA - 4MM G G A A A T T A G G T G C G C T T G G C A 0.008088917 0.007235897 0.009187739 0.009059137 0.01321546 0.012239587 0.01349002 0.009095838 0.008058686 0.004378313 0.004218266 0.004802849 0.009711245 0.006689238 0.004530909 G 0.011665167 0.01104882 0.011660399 0.013118698 0.013854333 0.005662421 0.010356339 0.011943719 0.014278694 0.006185748 0.007451365 0.007873612 0.005389083 0.00341051 0.004032071 0.009297337 0.005416372 C 0.012097026 0.010209726 0.012565488 0.010991471 0.01336796 0.00968052 0.008081519 0.004886894 0.00338281 0.00574918 0.004733436 0.004490072 T 0.003554228 0.012469263 0.012857257 0.009754599 0.012891079 0.009797765 0.005934739 0.006437405 0.007650772 0.006868489 0.004592492 0.007550738 0.00887784 0.010053937 0.00730103 0.006237737 Sp - Nmax- 4MM G G A A A T T A G G T G C G C T T G G C A 0.663725206 0.546746869 0.696531905 0.753592893 0.903086641 0.906382995 0.927860102 0.697967836 0.604745053 0.493138513 0.491366036 0.530722802 0.758104275 0.613484142 0.529291206 G 0.843289552 0.759271977 0.830640924 0.894034911 0.94765514 0.506516787 0.93724394 0.799387189 1 0.61371202 0.667964145 0.654011863 0.577381736 0.497354257 0.519424756 0.727681958 0.533807471 C 0.864324621 0.699048404 0.815798079 0.768319341 0.937881283 0.722721613 0.69811234 0.568650543 0.506282437 0.578621426 0.536895379 0.592912107 T 0.480177913 0.810838435 0.856659362 0.742124542 0.913990926 0.774922297 0.510647659 0.586155968 0.772289197 0.645998681 0.496302274 0.59513261 0.748722437 0.625553398 0.58107564 0.602643816 Sp - ΔRA - 5MM G G A A A T T A G G T G C G C T T G G C A 0.007768107 0.004688037 0.00652621 0.006606571 0.008276048 0.006597949 0.006017228 0.004874914 0.004039075 0.005376428 0.004000008 0.003335793 0.011655882 0.004577908 0.003823652 G 0.006802268 0.006158693 0.005578589 0.016887517 0.008253837 0.006346893 0.007777778 0.006403983 0.006269484 0.005348714 0.005813487 0.005457523 0.004830988 0.003746343 0.004703613 0.00444499 0.004247639 C 0.008498533 0.006734113 0.007482033 0.017250731 0.017870204 0.006259515 0.004657503 0.002809934 0.002423775 0.003400124 0.002941875 0.00374167 T 0.006599483 0.009492764 0.009299193 0.008589996 0.009187592 0.007273922 0.004940252 0.007053772 0.004686806 0.015206387 0.00269224 0.004407122 0.004721836 0.005565213 0.004812354 0.003721261 Sp - Nmax- 5MM G G A A A T T A G G T G C G C T T G G C A 0.570864506 0.386074586 0.506354725 0.535714151 0.605819212 0.520563934 0.485587386 0.413037989 0.381835248 0.430104426 0.379770676 0.353987055 0.698670037 0.441291271 0.391232514 G 0.548050582 0.509803385 0.484464195 0.904815669 0.616881016 0.484556148 0.596671508 0.498138207 0.518478299 0.451851396 0.487394702 0.458356848 0.417827854 0.352340928 0.437968405 0.423102362 0.392888675 C 0.632250912 0.549534518 0.572123989 0.970047877 1 0.518020475 0.457590455 0.350919576 0.34146698 0.403028188 0.366953572 0.435605921 T 0.567562991 0.640727522 0.644943972 0.595577413 0.618654774 0.555220775 0.399237354 0.523166758 0.436451271 0.847886684 0.324701569 0.405314338 0.461756771 0.508292995 0.441139222 0.419338636 Sp - ΔRA - 6MM G G A A A T T A G G T G C G C T T G G C A 0.009219498 0.005271722 0.007015904 0.007781036 0.008328668 0.006622262 0.005985072 0.004922225 0.004373754 0.006286001 0.004580737 0.00441761 0.006717549 0.006229974 0.004531291 G 0.007224468 0.006662713 0.007264402 0.010303526 0.007728283 0.006401696 0.00911484 0.006435644 0.004443285 0.005298349 0.008012687 0.005095754 0.0049918 0.004417989 0.005138159 0.005248414 0.004531044 C 0.008248147 0.008219458 0.007622755 0.009575076 0.010171512 0.00707182 0.005065518 0.003291177 0.003331136 0.005146007 0.005266321 0.004094472 T 0.010220444 0.009270455 0.009193463 0.008717424 0.010224692 0.00837517 0.005232156 0.008777666 0.004818844 0.009932735 0.002651531 0.004634108 0.004141875 0.006700812 0.005011395 0.004671883 Sp - Nmax- 6MM G G A A A T T A G G T G C G C T T G G C A 0.824187047 0.52773983 0.678805474 0.736810928 0.809455079 0.708428962 0.680314778 0.638681657 0.533578706 0.718931288 0.584408792 0.603335638 0.726456446 0.689181617 0.632513649 G 0.764536469 0.729636418 0.746587581 0.815438863 0.760963301 0.624848728 0.798515333 0.738688568 0.594283707 0.593680295 0.724418315 0.609773558 0.674698842 0.569878002 0.658583406 0.603534922 0.597001644 C 0.785045024 0.760045434 0.756625072 0.902070283 0.843189811 0.783909789 0.66541689 0.532748773 0.525870867 0.585649325 0.650596195 0.5887472 T 0.917530582 1 0.932622241 0.81657246 0.833185597 0.785429119 0.577047752 0.791651077 0.66710085 0.841063623 0.504123049 0.56154374 0.62812806 0.725342844 0.599204973 0.57451614 ===== > SaCas9 Sa - ΔRA - 1MM G G A A A T T A G G T G C G C T T G G C A -0.004385486 -0.004320323 -0.005745192 -0.00338546 -0.000821428 -0.000793582 -0.001137751 -0.006325162 -0.008377872 -0.010620867 -0.0025174 -0.002063339 0.007512794 -9.6681E-05 -0.008568772 G -0.006004047 -0.007480681 -0.004013692 -0.004604665 -0.002738731 -0.00248916 -0.001637628 -0.001621909 -0.009215283 -0.004614541 -0.048693623 -0.012087624 -0.005828022 -0.010226443 -0.003274893 0.008177064 -0.002587549 C -0.006323366 -0.003290895 -0.002258543 -0.00323165 -0.00426982 -0.000667659 -0.007252016 -0.006138858 -0.005646982 -0.005803332 -0.005417918 -0.009421691 T -0.004995254 -0.009366698 -0.00465065 -0.003384127 -0.019984732 -0.041054907 -0.008271074 -0.039263547 -0.011222774 -0.004722036 -0.004358677 -0.004779663 -0.009031289 0.003539032 -0.002117096 -0.003777191 Sa - Nmax- 1MM G G A A A T T A G G T G C G C T T G G C A 0.140931462 0.120404196 0.156857133 0.13414002 0.113402866 0.113992835 0.133274078 0.180322472 0.241769671 0.270167812 0.095354885 0.104126828 0.403057861 0.118211315 0.215369087 G 0.163155566 0.189377474 0.134833052 0.197921385 0.151103059 0.09208712 0.103911029 0.136503277 0.239251869 0.145293734 1 0.316316997 0.168440244 0.263345889 0.134167319 0.455751747 0.171361352 C 0.177128348 0.1255906 0.101834758 0.137471827 0.174127925 0.092754563 0.230036095 0.18787845 0.192185027 0.176145109 0.217909151 0.378304712 T 0.161613083 0.240684048 0.202070092 0.128136728 0.517503348 0.860356854 0.209209217 0.802488039 0.287026335 0.144006864 0.143776754 0.148955606 0.243312987 0.206535854 0.21716176 0.124090554 Sa - ΔRA - 2MM G G A A A T T A G G T G C G C T T G G C A -0.005197676 -0.005493762 -0.006197993 -0.001023394 0.005211964 0.005397266 0.006502754 0.002240005 -0.001567871 -0.008634603 -0.002266608 0.000881697 0.00474671 0.002791405 -0.013740878 G -0.003320665 -0.009632095 -0.001787291 0.001168511 0.007726985 -0.008381335 0.001110254 0.005648952 -0.002989051 -0.009083052 -0.01962389 -0.004923887 -0.004244334 -0.0236187 -0.014517585 0.008566866 0.001788795 C -0.007813372 -0.002304401 -0.003820746 0.000827229 0.003483662 0.000869359 0.002383301 -0.00308352 -0.001954732 -0.004772644 -0.000935868 0.009831386 T -0.006059017 -0.010701032 0.001683382 -0.001287362 0.000784628 -0.018603422 -0.006304036 -0.021366144 -0.009654199 -0.012727514 -0.007182717 -0.013463673 -0.005046846 0.007224186 0.005619983 -0.003479755 Sa - Nmax- 2MM G G A A A T T A G G T G C G C T T G G C A 0.265779395 0.269609609 0.306602957 0.252346944 0.317552413 0.41698295 0.404511274 0.36654201 0.34720475 0.38140959 0.210595644 0.201809935 0.668178039 0.22357195 0.48535367 G 0.306566828 0.422654164 0.300080827 0.318449922 0.382307715 0.300171773 0.236368764 0.412208069 0.386735984 0.324596324 1 0.412967278 0.298563004 0.631983745 0.454058105 0.61702525 0.302070436 C 0.376828177 0.257151503 0.259126613 0.231851941 0.336911492 0.257165009 0.367565702 0.307147387 0.344559483 0.332101695 0.489541139 0.650642748 T 0.292055043 0.429808584 0.366586292 0.22602417 0.584827087 0.98299523 0.348028263 0.818493274 0.453595046 0.37532535 0.312073498 0.398782893 0.404923245 0.360883485 0.433979569 0.29418742 Sa - ΔRA - 3MM G G A A A T T A G G T G C G C T T G G C A 0.003479419 0.002624638 0.004821226 0.007388871 0.008107798 0.009014244 0.010955928 0.012318964 0.010218563 0.003627967 0.004623016 0.005379253 0.005550752 0.004046601 0.004398254 G 0.005438714 0.003988556 0.006351172 0.008210951 0.009881789 -0.004343974 0.008342853 0.009592335 0.010624834 0.003066402 0.009972746 0.009021568 0.007019618 -0.00853251 -0.012064932 0.00508047 0.006639635 C 0.003564813 0.006478989 0.00605513 0.007622342 0.010813193 0.007081414 0.012503578 0.007707122 0.005206517 0.006477902 0.007984801 0.01821004 T 0.003552775 0.004281175 0.011254489 0.006589482 0.01367664 0.009966868 0.005784818 0.003184665 0.003767845 -0.001912599 0.003045714 -0.003814623 0.00894418 0.005537148 0.009925464 0.006259045 Sa - Nmax- 3MM G G A A A T T A G G T G C G C T T G G C A 0.419870215 0.38266476 0.495472215 0.564669347 0.62860625 0.671614907 0.781237505 0.702466338 0.613458609 0.39845548 0.372284124 0.435062979 0.476004906 0.314100068 0.541635124 G 0.572837422 0.558185434 0.584627957 0.615800762 0.695445445 0.383084193 0.553764313 0.732033545 0.711296757 0.38490331 0.833279497 0.570855154 0.462518236 0.583062021 0.572651538 0.479963543 0.350014804 C 0.543750913 0.530781221 0.528452056 0.635002025 0.805296786 0.55321228 0.756018073 0.558997218 0.42523808 0.399011905 0.47508848 1 T 0.371258217 0.509962809 0.653038455 0.525150952 0.785184327 0.814370718 0.493925654 0.628282879 0.450905034 0.383187156 0.367577886 0.418897453 0.496033931 0.375398843 0.498843209 0.471648026 Sa - ΔRA - 4MM G G A A A T T A G G T G C G C T T G G C A 0.008561963 0.007110173 0.008286171 0.008403118 0.006874613 0.007159504 0.008688591 0.009372506 0.008441407 0.008937051 0.006223892 0.004762593 0.00305966 0.004591342 0.011796721 G 0.009295006 0.009257417 0.008741856 0.009335325 0.008620503 0.005576867 0.010712198 0.00773517 0.012142704 0.008274296 0.016599215 0.012444584 0.009163696 0.009587864 0.003560719 0.00157417 0.005115689 C 0.009847012 0.009604894 0.010496037 0.008570369 0.010605813 0.007934319 0.012984597 0.006844805 0.005227777 0.010276428 0.005736278 0.01092339 T 0.009301133 0.012736483 0.012313766 0.009514238 0.015948557 0.01761747 0.010152274 0.013379706 0.010184797 0.009460534 0.008284316 0.009248562 0.013080253 0.003557202 0.010833256 0.00779692 Sa - Nmax- 4MM G G A A A T T A G G T G C G C T T G G C A 0.674926729 0.512593827 0.63390739 0.694499383 0.573651103 0.576423708 0.657252749 0.623873979 0.559020989 0.643182625 0.49803564 0.468347866 0.334376719 0.440819559 0.736298362 G 0.745076342 0.701647046 0.628061138 0.693026888 0.680030498 0.582163391 0.825694017 0.611696227 0.803629348 0.521829376 1 0.79363842 0.676835224 0.641799967 0.58720845 0.260177715 0.436845954 C 0.777079109 0.70641587 0.774276529 0.755270954 0.846325891 0.677597785 0.831534795 0.558534465 0.443329417 0.62534767 0.478660533 0.719042965 T 0.639605544 0.818035115 0.913477292 0.728131486 0.938747815 0.991698186 0.628801064 0.8192271 0.788311333 0.71490205 0.542016624 0.624242 0.72026954 0.318261497 0.69324836 0.559915837 Sa - ΔRA - 5MM G G A A A T T A G G T G C G C T T G G C A 0.006205034 0.004312938 0.00573035 0.004240534 0.003839303 0.004337233 0.003512736 0.00311639 0.002939097 0.005879298 0.003354566 0.002711671 0.000317703 0.002366901 0.006345182 G 0.005804451 0.005805828 0.004885581 0.003797863 0.004758026 0.007300368 0.005275601 0.002990968 0.005491612 0.004455062 0.008026046 0.00601157 0.004422904 0.009311489 0.008426451 0.000480762 0.003265692 C 0.0078069 0.005833101 0.006562645 0.004230574 0.00418656 0.003920961 0.00594105 0.002425789 0.001897354 0.004192524 0.002281324 0.003153497 T 0.007506035 0.01002968 0.00787857 0.005571302 0.008445967 0.009013211 0.005827491 0.009827383 0.006604378 0.006449481 0.003456222 0.007957095 0.006533701 0.001011168 0.00565806 0.003269074 Sa - Nmax- 5MM G G A A A T T A G G T G C G C T T G G C A 0.492263022 0.35795103 0.422412333 0.399115421 0.362995294 0.352095085 0.340985767 0.28320403 0.269658215 0.440402429 0.2929179 0.259536257 0.592467235 0.263880809 0.426797786 G 0.499892176 0.466312384 0.417565207 0.914208634 0.425453639 0.544044902 0.46560868 0.341185254 0.427809539 0.32876376 0.527435666 0.471315337 0.375709949 0.549956079 0.498330616 0.130380222 0.304529919 C 0.53887926 0.472365051 0.507950097 0.967237693 1 0.367791295 0.44214314 0.287242247 0.228250993 0.361704457 0.285471861 0.31900923 T 0.540228238 0.656535489 0.59649956 0.528923776 0.592920875 0.594503529 0.431961073 0.592524971 0.473227314 0.975123358 0.320658371 0.508543825 0.467320737 0.197781996 0.413009335 0.3121326 Sa - ΔRA - 6MM G G A A A T T A G G T G C G C T T G G C A 0.008122971 0.005596831 0.004970749 0.0055641 0.004982218 0.005149849 0.005694563 0.002560116 0.001679557 0.006511549 0.00153379 0.001410664 0.001395369 0.002285877 0.005098987 G 0.007680689 0.00479572 0.005608428 0.00720015 0.00719092 0.007302686 0.006282171 0.001158776 0.003173195 0.003779971 0.00752134 0.003514452 0.003608383 0.011626496 0.011311692 0.000140671 0.001787061 C 0.00967296 0.006771011 0.006081988 0.005544424 0.004727348 0.005116213 0.005110285 0.001282884 0.002616396 0.002774568 0.001605999 0.000565817 T 0.007901398 0.010533309 0.00977779 0.006661337 0.007994109 0.009220607 0.006387362 0.011816069 0.00534383 0.008459038 0.003943441 0.00625351 0.004859394 0.001034328 0.005388458 0.002416174 Sa - Nmax- 6MM G G A A A T T A G G T G C G C T T G G C A 0.814385632 0.553286996 0.629551459 0.614426635 0.555005242 0.543547029 0.569977381 0.438052321 0.40053762 0.645113456 0.383547972 0.395597841 0.366584285 0.419697945 0.599992261 G 0.65638308 0.693597919 0.617267033 0.774522407 0.612285483 0.726878813 0.66043237 0.514738114 0.488973856 0.468932851 0.69982877 0.54914631 0.544792111 0.738571415 0.744491669 0.270360251 0.37962357 C 0.852385731 0.680253176 0.712240497 0.80480552 0.713489483 0.602862512 0.614331399 0.381042835 0.355984463 0.496697939 0.433740418 0.374277477 T 0.859192853 1 0.861192388 0.722916419 0.822986108 0.817045375 0.63739331 0.940264716 0.65915971 0.907359165 0.455348885 0.644249395 0.594350455 0.30390324 0.538683786 0.412803675 ===== > SpCas9 HF1 HF1 - ΔRA - 1MM G G A A A T T A G G T G C G C T T G G C A 0.007878928 -0.004681273 0.005552985 0.007833976 0.020399802 0.013367822 0.010549782 -0.012022204 0.003344742 -0.012110936 -0.017124956 -0.008766887 0.003511242 0.001197784 -0.004707266 G 0.006408014 0.00488408 0.006722143 0.010924157 0.020774535 -0.013285614 0.010868469 0.00137121 0.01560522 -0.022958513 -0.011630059 0.008915933 -0.022175763 -0.017903073 -0.036621731 0.006940822 -0.005514553 C 0.007789202 -0.000783855 0.006672348 -0.000760123 0.013996131 -0.007540926 -0.013628368 -0.002126421 -0.00372683 0.001922753 0.002443721 0.001725082 T -0.007619025 0.003318318 0.021779781 0.009679886 0.01208239 -0.009510038 -0.010212928 0.002346965 0.00640656 0.005948655 -0.004015055 0.009660706 0.003681955 0.00256706 0.001807359 -0.000989668 HF1 - Nmax- 1MM G G A A A T T A G G T G C G C T T G G C A 0.370943599 0.295136443 0.396770346 0.375602073 0.795185335 0.550953527 0.495196938 0.34149038 0.44967393 0.378818748 0.50078772 0.40252341 0.458550531 0.281430089 0.2569969 G 0.296577792 0.292791326 0.282749115 0.449415064 0.77500296 0.389674289 0.85641531 0.592161151 0.779967054 0.525791237 0.440848737 0.454704289 0.629571364 0.495832674 0.999941971 0.481065859 0.325234307 C 0.332713976 0.323161457 0.536443529 0.455094971 0.763727199 0.441823535 0.409065859 0.450511888 0.427927056 0.321731954 0.222951793 0.380170505 T 0.259552528 0.336935717 1 0.446063633 0.69031008 0.533000107 0.459700918 0.864066189 0.498977091 0.607604123 0.327330094 0.473147677 0.287855211 0.236230224 0.389033071 0.417219349 HF1 - ΔRA - 2MM G G A A A T T A G G T G C G C T T G G C A 0.009431557 0.005307727 0.011707497 0.011489796 0.020277075 0.01373551 0.013014691 0.002771227 0.013948102 0.001472521 0.005629013 0.008349308 0.00586 0.005826382 0.003916471 G 0.010851014 0.007006029 0.009976073 0.017016011 0.023221739 -0.00186628 0.01441891 0.014376155 0.021255128 -0.002288423 0.00613233 0.009652099 0.005542134 0.002069322 0.00588081 0.006659166 0.005554592 C 0.011765439 0.008739692 0.019955896 0.010815336 0.017081037 0.008682658 0.001029457 0.010096813 0.008094738 0.00520178 0.005381332 0.005812917 T -0.002843328 0.007157051 0.020618906 0.012242133 0.012387147 0.007763774 0.003541814 0.01289151 0.014123976 0.01268185 0.006180451 0.010170568 0.005062906 0.003623699 0.006230165 0.0137293 HF1 - Nmax- 2MM G G A A A T T A G G T G C G C T T G G C A 0.425289002 0.368931679 0.496817092 0.491869565 0.97672718 0.704004355 0.691763844 0.427189168 0.695306157 0.405259285 0.537439736 0.450866526 0.45295806 0.346411796 0.382015579 G 0.470416126 0.407036262 0.491884514 0.750935246 1 0.363224903 0.699705212 0.73536612 0.928585896 0.525252722 0.482806853 0.50788435 0.517614021 0.479858982 0.884670109 0.467502061 0.393121077 C 0.533633014 0.431178932 0.767824107 0.637135033 0.825978636 0.608561553 0.494401265 0.794409286 0.425307445 0.372327589 0.324030576 0.547895182 T 0.33054912 0.451048813 0.823277714 0.583637332 0.570305313 0.527399778 0.451578228 0.738875817 0.724825532 0.538197199 0.385469344 0.47646291 0.452888687 0.315901859 0.400899463 0.772763884 HF1 - ΔRA - 3MM G G A A A T T A G G T G C G C T T G G C A 0.009260422 0.007066759 0.010761661 0.01004371 0.016462125 0.011864534 0.01070504 0.010566523 0.010321961 0.00981899 0.012056027 0.009786271 0.004198288 0.006315493 0.010253912 G 0.009538616 0.009787164 0.009290803 0.011469299 0.014760011 0.008547117 0.018549076 0.010813869 0.014240519 0.012245462 0.011856759 0.009677825 0.013011849 0.009072854 0.014322978 0.004640842 0.007490622 C 0.010234099 0.011835583 0.017498182 0.014195147 0.014241621 0.01222598 0.015110892 0.006873846 0.00567126 0.005346431 0.003117563 0.00845795 T 0.006086871 0.006486936 0.017728246 0.01100262 0.012339513 0.01117215 0.011485964 0.015548001 0.011170009 0.012783117 0.007172215 0.008600257 0.003796633 0.003647816 0.00876713 0.011255171 HF1 - Nmax- 3MM G G A A A T T A G G T G C G C T T G G C A 0.628984096 0.505043599 0.678548033 0.619874887 0.950797968 0.7532564 0.79663338 0.67519131 0.731606656 0.582036497 0.686134719 0.650524977 0.385923668 0.511011205 0.673490327 G 0.612782828 0.596927931 0.622594176 0.744903802 0.891122544 0.609468449 0.957192046 0.754954163 0.898536311 0.733734591 0.734681691 0.71992312 0.726002972 0.589995897 0.724757509 0.395425624 0.603868238 C 0.657803839 0.665997435 0.899051446 0.844173327 0.922835098 0.69842168 0.808654784 0.605358503 0.454669413 0.501655254 0.390640752 0.681436652 T 0.449407229 0.587110281 1 0.792846174 0.785482378 0.72136472 0.676923079 0.736456217 0.7959273 0.759666424 0.556840299 0.693753276 0.406075192 0.304458881 0.624204207 0.847433559 HF1 - ΔRA - 4MM G G A A A T T A G G T G C G C T T G G C A 0.008843231 0.008489212 0.008998354 0.009457982 0.010484457 0.006589547 0.00667738 0.009179366 0.004283846 0.011761685 0.008736973 0.005625021 0.003624292 0.004568527 0.009573261 G 0.009061543 0.008987796 0.008283405 0.009849346 0.010403837 0.011014366 0.011674709 0.006388633 0.00738646 0.01015215 0.010901346 0.005287534 0.010848108 0.007646632 0.011855679 0.003968892 0.005809676 C 0.009689149 0.010226075 0.0143175 0.010341002 0.008745738 0.006827473 0.010631008 0.002805635 0.002823525 0.004438797 0.001289821 0.004854401 T 0.010970636 0.00673023 0.013045998 0.007886466 0.008298429 0.010144294 0.010092655 0.014335596 0.006282838 0.01025294 0.004731056 0.006446039 0.002817903 0.003056372 0.007750571 0.006137422 HF1 - Nmax- 4MM G G A A A T T A G G T G C G C T T G G C A 0.744307098 0.67074271 0.738821818 0.756860228 0.805439156 0.572433145 0.616535482 0.665658878 0.500062194 0.771622531 0.701128187 0.524259091 0.415929614 0.517630324 0.728570165 G 0.721406005 0.701940632 0.629798632 0.777666109 0.783764825 0.783826881 0.809488216 0.485230885 0.591210746 0.722595926 0.781543843 0.585001143 0.777201839 0.576646716 0.742198762 0.365501836 0.558060007 C 0.736384475 0.734177825 0.923945711 0.7523178 0.659324003 0.545356274 0.739132172 0.36061116 0.3476492 0.412805238 0.303511101 0.538725139 T 0.790174648 0.678896051 1 0.75090785 0.785432639 0.793189652 0.780685827 0.940712595 0.580343943 0.819612189 0.484346539 0.60096869 0.370941419 0.379713012 0.713090959 0.646485726 HF1 - ΔRA - 5MM G G A A A T T A G G T G C G C T T G G C A 0.008633685 0.011963359 0.010224763 0.009398695 0.008357116 0.007148698 0.005223176 0.004273433 0.00246266 0.007646107 0.005795334 0.004255199 0.004679515 0.004201492 0.006227486 G 0.007327297 0.007338003 0.007569009 0.009307351 0.00976868 0.007562356 0.00678865 0.004690917 0.00432854 0.007351348 0.007521747 0.003516357 0.007591753 0.005104057 0.010056315 0.004510998 0.004365427 C 0.009539083 0.0089218 0.012030615 0.008688198 0.006256445 0.004214139 0.008349361 0.001156182 0.002374387 0.003079079 0.001267283 0.004622613 T 0.01152043 0.007437222 0.008949116 0.007945125 0.006042365 0.006714629 0.009626048 0.012050606 0.005238455 0.007548583 0.002875826 0.004764416 0.003458681 0.003890071 0.004414054 0.005631387 HF1 - Nmax- 5MM G G A A A T T A G G T G C G C T T G G C A 0.793688787 0.820118267 0.807469689 0.787242646 0.761329892 0.575387775 0.570030656 0.539260773 0.459703388 0.701057596 0.588389089 0.480187127 0.450417648 0.534126812 0.660509489 G 0.723992568 0.741073651 0.689803512 0.731522746 0.806926886 0.742767473 0.644375294 0.48104679 0.55798517 0.601366615 0.668888289 0.510830451 0.652681899 0.505248816 0.71533737 0.451490819 0.542161417 C 0.820085689 0.747112499 0.86986872 0.726894775 0.59116156 0.465422145 0.619324074 0.379874323 0.382846381 0.454952407 0.389988384 0.574116582 T 1 0.770935436 0.848279503 0.741587722 0.688038898 0.690070357 0.784253473 0.894566961 0.543144441 0.711845772 0.464258626 0.586795651 0.413599862 0.545417937 0.611274335 0.616914522 HF1 - ΔRA - 6MM G G A A A T T A G G T G C G C T T G G C A 0.00625293 0.010524832 0.009735829 0.008683272 0.005753618 0.007509586 0.005755058 0.002743416 0.004508965 0.004926709 0.004842559 0.003476481 0.002039513 0.002142333 0.004988697 G 0.007594409 0.004196074 0.005869909 0.005784116 0.006396142 0.005816139 0.006020412 0.003913518 0.00132809 0.004767999 0.004643131 0.002687697 0.004323018 0.00303355 0.006023021 0.004802977 0.00620913 C 0.006536029 0.007194135 0.005767748 0.008464854 0.007514769 0.001512207 0.007869521 0.003639894 0.001517509 0.003780823 0.001162329 0.00316957 T 0.007961237 0.00560403 0.006607487 0.006210821 0.003796002 0.006824618 0.008745722 0.008697315 0.001228614 0.007026759 0.004574053 0.007243436 0.004074809 0.00480154 0.004473538 0.003563413 HF1 - Nmax- 6MM G G A A A T T A G G T G C G C T T G G C A 0.778308282 1 0.860665232 0.867926963 0.831922334 0.840393301 0.789009652 0.683606511 0.549236038 0.792574862 0.667798611 0.517150455 0.631816683 0.646549192 0.700650357 G 0.817784942 0.773022909 0.843511123 0.824186501 0.792115679 0.678740944 0.697973689 0.615006115 0.481858672 0.647107755 0.67894583 0.593734691 0.705829374 0.607734107 0.771436174 0.596627174 0.608551154 C 0.791807479 0.818137349 0.89317807 0.856969726 0.704504735 0.581058287 0.663836911 0.512576988 0.545315285 0.627979697 0.533114416 0.618844729 T 0.968229538 0.827852705 0.844378962 0.861008369 0.674076205 0.733853842 0.89976147 0.914106082 0.619696322 0.7355824 0.652827662 0.725687265 0.626997851 0.589028733 0.614386617 0.648726956 ===== > HypaCas9 Hypa - ΔRA - 1MM G G A A A T T A G G T G C G C T T G G C A 0.003057461 0.002549651 0.004991768 0.002353054 0.034791525 0.023088357 0.020254533 0.004048474 0.003446238 0.004164922 -0.000852652 0.003039877 0.025571076 0.005747242 0.002552919 G 0.006933686 0.003055787 0.012146754 0.02838862 0.035843446 0.000422393 -8.37809E-05 0.010882889 0.038176797 -0.010102087 -0.005025594 -0.002425106 0.000585323 0.002031681 -0.007053771 0.027373536 0.005532405 C 0.00723902 0.006019507 0.005965487 0.01078769 0.004596297 0.004268811 -0.000513132 0.007591803 0.002354078 0.00315446 0.006642812 0.004751291 T 0.005174807 0.010322709 0.010717061 0.003143772 0.033325894 0.008161327 -0.003009367 -0.003782697 0.012806356 0.001729404 0.0040635 0.004301863 0.015857454 0.027574922 -0.003294518 0.003417217 Hypa - Nmax- 1MM G G A A A T T A G G T G C G C T T G G C A 0.351658944 0.299578096 0.411029688 0.364006697 1 0.693021496 0.589979151 0.447712826 0.471853183 0.25833276 0.307001018 0.373055027 0.706234175 0.50792337 0.319851565 G 0.363525944 0.319186881 0.55627249 0.900140256 0.999040954 0.262052044 0.529203078 0.651862242 0.962401309 0.5485898 0.337571466 0.369921566 0.373983109 0.360130545 0.558276876 0.734906161 0.504442214 C 0.445307398 0.438236454 0.52183471 0.54767095 0.600661636 0.459353875 0.45474308 0.503867832 0.488092174 0.5640724 0.500885595 0.46973629 T 0.239728727 0.473071599 0.721878458 0.478690546 0.956360832 0.548693629 0.303717858 0.401956334 0.601350327 0.438804414 0.437767979 0.4596983 0.594436487 0.788316642 0.333253913 0.529227812 Hypa - ΔRA - 2MM G G A A A T T A G G T G C G C T T G G C A 0.007915462 0.007078285 0.009659315 0.008995183 0.020899602 0.014072807 0.013791022 0.011478997 0.012872592 0.006332119 0.008408104 0.007053175 0.011495589 0.01001611 0.007355899 G 0.008616402 0.008721873 0.014270639 0.019100724 0.022692622 0.004461824 0.013926067 0.014827069 0.021435349 0.014083787 0.009413507 0.009089186 0.009316706 0.005881489 0.011095041 0.013699482 0.009882859 C 0.011313176 0.011702512 0.017023671 0.011850033 0.017998976 0.009147286 0.015308112 0.00993468 0.006558961 0.009355141 0.008601299 0.009191843 T 0.002956079 0.008703053 0.016051402 0.010957817 0.015942804 0.012712457 0.007804421 0.006780658 0.013389362 0.010350361 0.007519612 0.010615906 0.012008173 0.012186047 0.006896509 0.01197888 Hypa - Nmax- 2MM G G A A A T T A G G T G C G C T T G G C A 0.278996186 0.267857873 0.320890179 0.316023984 0.512318348 0.62382991 0.669038239 0.43972042 0.449896739 0.301515377 0.32910693 0.287780143 0.47459329 0.32081112 0.262072328 G 0.28816005 0.310344573 0.381029134 0.481463179 0.602996379 0.266199206 0.439905469 0.612151564 1 0.51263422 0.39194163 0.300028717 0.346029643 0.278039565 0.555384627 0.438334155 0.324487874 C 0.355816411 0.379348973 0.485280874 0.370994983 0.569321157 0.345218787 0.456460537 0.435704399 0.261056474 0.324277767 0.297279767 0.355899355 T 0.206477647 0.287189503 0.472999863 0.36613143 0.498226236 0.382550028 0.338229984 0.380355097 0.466346792 0.367353952 0.289727851 0.352140261 0.439384634 0.340631233 0.295912394 0.421800613 Hypa - ΔRA - 3MM G G A A A T T A G G T G C G C T T G G C A 0.014829364 0.013691781 0.016763749 0.015387679 0.024993282 0.01385832 0.012800496 0.014352453 0.015040653 0.016659584 0.014763493 0.011723297 0.01162667 0.01075627 0.012480638 G 0.015817045 0.016994482 0.019577687 0.021933787 0.023228277 0.01478553 0.022026671 0.016003656 0.01593356 0.025253217 0.020280348 0.014936341 0.0163919 0.012359585 0.020921103 0.015567638 0.01223391 C 0.018146056 0.020203212 0.025337075 0.020633469 0.02239885 0.014048331 0.023598645 0.009395079 0.006724047 0.010819939 0.007949145 0.011943661 T 0.011829657 0.014354828 0.024046472 0.017446986 0.016661844 0.014919513 0.018963197 0.020616223 0.013362401 0.017204771 0.010626113 0.014514966 0.010305765 0.012164762 0.013644154 0.01420565 Hypa - Nmax- 3MM G G A A A T T A G G T G C G C T T G G C A 0.569480426 0.482999316 0.577992468 0.590714212 0.916345231 0.798249705 0.84935203 0.706009766 0.665172382 0.521776169 0.561097768 0.45468512 0.624070536 0.554229402 0.505054782 G 0.581167538 0.55731129 0.681278529 0.812876679 0.897164622 0.561954062 0.956180677 0.823161346 0.968421638 0.94156994 0.81723012 0.686855791 0.610967302 0.43271747 0.580390082 0.688458523 0.566491083 C 0.626568994 0.64439045 0.834501311 0.746415715 1 0.63357125 0.811455047 0.444041809 0.314519555 0.461174197 0.404047571 0.565662613 T 0.369901747 0.596934708 0.928874727 0.784494042 0.950572885 0.783617485 0.690968054 0.674094937 0.678153478 0.662265998 0.432217229 0.644587676 0.623665274 0.574899462 0.581093463 0.707839763 Hypa - ΔRA - 4MM G G A A A T T A G G T G C G C T T G G C A 0.016685138 0.016787422 0.017800993 0.018700153 0.019534338 0.010215376 0.009439294 0.010525332 0.009207148 0.017389981 0.011968666 0.008296228 0.010823832 0.008625945 0.012115002 G 0.017566307 0.018981051 0.017857773 0.019677548 0.019059824 0.016428169 0.017115286 0.008862278 0.009457637 0.018560627 0.019171052 0.011856313 0.014835522 0.009859032 0.019041151 0.012138579 0.009514801 C 0.018344603 0.020877703 0.024627335 0.017269794 0.013717377 0.010754926 0.017549365 0.005421856 0.005106313 0.008407177 0.004980818 0.008615843 T 0.016928975 0.015659034 0.021338342 0.01714213 0.012597824 0.012785284 0.016413547 0.02210578 0.009494167 0.016193367 0.008273471 0.01090307 0.007585595 0.012484688 0.011894891 0.009400549 Hypa - Nmax- 4MM G G A A A T T A G G T G C G C T T G G C A 0.748663422 0.706047699 0.759916248 0.833793894 0.85833901 0.576399864 0.614218254 0.561173475 0.458845127 0.64377425 0.521621266 0.379452355 0.582835456 0.534681405 0.569103051 G 0.831561706 0.741998598 0.759291857 0.878912296 0.847125676 0.739804492 0.84548437 0.534890272 0.564543638 0.752137032 0.828513909 0.613654542 0.676791452 0.414545818 0.654474614 0.568121504 0.518639497 C 0.776170757 0.780125983 0.902890222 0.735808465 0.726250118 0.536549685 0.719402874 0.28218163 0.247362904 0.376969654 0.302226612 0.437470715 T 0.700301425 0.784006355 1 0.792561752 0.806223911 0.800123287 0.709480719 0.921875621 0.495381766 0.805562614 0.373111433 0.578381981 0.473346387 0.623880365 0.659859464 0.553346729 Hypa - ΔRA - 5MM G G A A A T T A G G T G C G C T T G G C A 0.019793615 0.019993537 0.020747664 0.018186922 0.017546479 0.01163056 0.009718154 0.007839292 0.009111638 0.016367435 0.010223096 0.008437979 0.010251466 0.008684686 0.009960003 G 0.017419934 0.020315932 0.018667897 0.019813841 0.021265963 0.015321576 0.012897772 0.009125161 0.007312163 0.012317626 0.014340644 0.009809791 0.013425573 0.008372828 0.016568147 0.013089588 0.009407383 C 0.02160272 0.019196512 0.022717581 0.018792516 0.014017451 0.00959264 0.012987472 0.005965323 0.004863754 0.007329657 0.006574791 0.008859757 T 0.021237609 0.016764743 0.020107327 0.017449268 0.012147346 0.011559498 0.016942639 0.018652272 0.009466748 0.014625199 0.008082648 0.011797888 0.009704884 0.013520908 0.010239832 0.008712348 Hypa - Nmax- 5MM G G A A A T T A G G T G C G C T T G G C A 0.922522276 0.926948625 0.884703873 0.913399557 0.918057642 0.614875899 0.616416873 0.475295195 0.412701502 0.66615379 0.488610884 0.371376332 0.57549434 0.546227695 0.549909242 G 0.906847381 0.938576931 0.884341082 0.923111662 0.971210295 0.795033894 0.723801735 0.500045399 0.55152485 0.622203351 0.722238956 0.560971963 0.653148927 0.406307006 0.634081531 0.614746085 0.522528972 C 0.987601872 0.885182384 0.953281727 0.798962851 0.672084058 0.491738065 0.558331625 0.319700603 0.307567198 0.407967426 0.345689446 0.468154668 T 1 0.912434956 0.977975075 0.852705171 0.727414822 0.684239833 0.751863772 0.920094194 0.484923102 0.778373228 0.418400796 0.575014553 0.535715022 0.787788003 0.584538462 0.527626987 Hypa - ΔRA - 6MM G G A A A T T A G G T G C G C T T G G C A 0.01649888 0.019832702 0.020315046 0.018787701 0.015971159 0.016009311 0.010365898 0.008372968 0.008764708 0.013881247 0.007631075 0.006095448 0.011063876 0.008897626 0.009089701 G 0.017355333 0.014866303 0.014456346 0.01526752 0.016039146 0.014009442 0.012397873 0.008043999 0.007045716 0.010005308 0.010793687 0.010928877 0.015088463 0.011187994 0.013419852 0.013623273 0.010359341 C 0.017410753 0.016946704 0.019221827 0.015090532 0.014658613 0.010092198 0.013887753 0.00482739 0.007881377 0.006914802 0.007504659 0.005473802 T 0.016664551 0.015148663 0.01592899 0.018045994 0.011125542 0.009682175 0.019486357 0.017653896 0.007987086 0.013195785 0.009412097 0.012815347 0.010837551 0.014168655 0.008592888 0.008069012 Hypa - Nmax- 6MM G G A A A T T A G G T G C G C T T G G C A 0.894517803 1 0.914164896 0.935517952 0.866180958 0.827003602 0.736346336 0.555504202 0.464823428 0.674018331 0.504509596 0.434112306 0.557252568 0.583652452 0.564522965 G 0.94640517 0.899463106 0.827060509 0.889010082 0.886615251 0.738985001 0.727915189 0.548771326 0.525274715 0.575556095 0.634010271 0.527957929 0.615820078 0.481758673 0.639273663 0.553705498 0.563733366 C 0.846748261 0.807778642 0.855204851 0.786335933 0.632305154 0.511912505 0.591658776 0.401093543 0.430934988 0.453839711 0.479338277 0.454832152 T 0.913552063 0.946065062 0.925300829 0.94657728 0.670378647 0.725203627 0.862091967 0.896170207 0.524543493 0.776209199 0.568700252 0.732292561 0.536336784 0.869390294 0.568309928 0.545038643 ===== > HiFi Cas9 HiFi - ΔRA - 1MM G G A A A T T A G G T G C G C T T G G C A -0.001884996 0.002695882 -0.003362987 0.000368609 -0.007563589 -0.014763384 -0.018077311 -0.004813845 -0.001913991 0.000764463 -0.001377439 0.002057009 -0.00096006 -0.006649787 0.000779194 G -0.00360764 -0.00121381 0.001305085 0.000774377 -0.007134551 -0.001362548 -0.004132979 -0.001479308 -0.036500778 -0.000222783 -0.000228031 -0.000759811 -0.00063149 -0.002287809 -0.0012685 0.004227546 -0.003325604 C -0.001506076 -0.000694681 0.000617505 -0.001843446 -0.001379321 -0.001715123 0.000664626 -0.001923821 0.004397812 -0.001503518 -0.000898418 0.000644177 T 0.00130628 -0.000273729 -0.003327122 -0.001919074 -0.015816583 -0.003805727 -0.001061145 -0.000893721 -0.004722477 -8.37568E-05 0.00288272 -0.001266628 -0.007476373 -0.010675541 -0.004110703 -0.000703309 HiFi - Nmax- 1MM G G A A A T T A G G T G C G C T T G G C A 0.305418852 0.345075526 0.370649397 0.299097925 0.629744781 0.706227934 0.677294787 0.281226679 0.305202305 0.254557634 0.314263778 0.373518832 0.694125086 0.419525303 0.322447224 G 0.369337921 0.369743287 0.526438995 0.440857726 0.565797423 0.210433083 0.327784745 0.413159706 1 0.286569522 0.295949707 0.247539791 0.304194559 0.399674853 0.382723475 0.621612671 0.376625591 C 0.314735919 0.302205261 0.339722868 0.321872995 0.403016583 0.391694455 0.337891482 0.377078213 0.446917154 0.366463563 0.378819331 0.401643318 T 0.255548599 0.334526043 0.455859805 0.311159219 0.546890556 0.281436854 0.269258837 0.293863253 0.313483089 0.26979131 0.376930043 0.271802978 0.448681028 0.511578571 0.289871168 0.400164787 HiFi - ΔRA - 2MM G G A A A T T A G G T G C G C T T G G C A 0.003428027 0.006914408 0.002520652 -0.000602216 0.011694624 0.009057667 0.00902245 -0.000277599 0.001956907 -0.000405895 1.2794E-05 -0.000143577 0.015133555 0.001265367 0.002499294 G 0.007028504 0.010271383 0.014275634 0.011391839 0.013229053 -0.002023778 -0.005230947 0.00450791 0.009582963 -0.004415095 -0.003857723 0.000853826 -0.000839175 0.000234727 -0.005780272 0.016524949 0.001837 C 0.007479754 -0.000152657 0.004306229 0.004705383 0.004301039 0.00293219 -0.00084156 0.004188968 0.003261849 0.000653835 0.004402546 0.002709426 T -0.002202301 0.006468742 -0.000541094 0.000204138 -0.006068033 -0.002130995 -0.002768705 -0.005646329 0.002871604 -0.004081699 0.000384361 -0.003004161 0.006795095 0.00468974 0.001357933 0.00367544 HiFi - Nmax- 2MM G G A A A T T A G G T G C G C T T G G C A 0.457587412 0.441357161 0.564177547 0.437677211 0.857226081 0.785687662 0.747065239 0.48545511 0.515309006 0.341871685 0.361130175 0.342570522 0.733315102 0.490342432 0.316789102 G 0.519472782 0.527971001 0.615838205 0.631376233 0.845121142 0.319414424 0.576698164 0.578376909 1 0.533454513 0.437107821 0.397170915 0.391252328 0.321626071 0.512128002 0.684502308 0.467546979 C 0.577023552 0.458177946 0.661089361 0.501403586 0.616844716 0.504772274 0.484759635 0.453503805 0.352649833 0.442561167 0.430624711 0.427714831 T 0.331819503 0.442272107 0.633637209 0.515219512 0.706311223 0.541177539 0.416059125 0.534073215 0.557609572 0.46651828 0.369425223 0.470903516 0.627264533 0.684897805 0.44701111 0.447857255 HiFi - ΔRA - 3MM G G A A A T T A G G T G C G C T T G G C A 0.009382554 0.008005949 0.011753768 0.009136138 0.019737502 0.016715298 0.016213498 0.010501246 0.01112621 0.004457907 0.004633302 0.003726086 0.011863376 0.007898102 0.004929361 G 0.011503479 0.011255561 0.014272877 0.014688539 0.018672012 0.004403833 0.01186401 0.01375659 0.018712838 0.00933137 0.006820762 0.009680838 0.006332945 0.002668483 0.002238112 0.012620886 0.006886246 C 0.012092973 0.01051 0.015754478 0.011249727 0.015854314 0.009740996 0.01363276 0.006978199 0.003981615 0.006379965 0.005442727 0.006356937 T 0.004289344 0.0113647 0.012949161 0.011811003 0.014025749 0.010616612 0.008612804 0.00626319 0.009311952 0.00790153 0.004225598 0.008395438 0.010638818 0.012350325 0.006926036 0.009363642 HiFi - Nmax- 3MM G G A A A T T A G G T G C G C T T G G C A 0.483654167 0.457156442 0.55766441 0.47082671 0.885731797 0.891067888 0.882472304 0.601856989 0.544851082 0.356334552 0.350666559 0.317877679 0.609167297 0.42895318 0.327359982 G 0.604408447 0.566454343 0.654068922 0.660544677 0.833886052 0.327305204 0.706292491 0.774377603 1 0.590408548 0.491714938 0.502588568 0.398826289 0.261418747 0.386457741 0.636088078 0.402806138 C 0.548043671 0.466935984 0.696464489 0.573415178 0.769754107 0.526909308 0.65517186 0.404756459 0.249592111 0.342194309 0.3382771 0.434142685 T 0.331622108 0.560667137 0.705882112 0.612867759 0.781659746 0.613530772 0.466533632 0.474304389 0.561756719 0.467585556 0.313421764 0.467537704 0.61097429 0.498305854 0.412434889 0.536473929 HiFi - ΔRA - 4MM G G A A A T T A G G T G C G C T T G G C A 0.011923954 0.009749396 0.013762383 0.013280569 0.016591813 0.012353728 0.010431778 0.009473982 0.007993042 0.010535023 0.007471669 0.005296183 0.007873737 0.007228111 0.006853968 G 0.013713683 0.01215216 0.011311983 0.014399912 0.015958519 0.010917829 0.014621357 0.009558501 0.010627544 0.012116535 0.014213623 0.010168344 0.010442385 0.004222189 0.009244815 0.008999905 0.006509701 C 0.01307191 0.013944633 0.017023714 0.013754074 0.013980665 0.009244648 0.012688149 0.003793654 0.002987429 0.004883652 0.003202184 0.006117828 T 0.012095467 0.012783408 0.018608593 0.013767788 0.015886828 0.013950467 0.012363062 0.015378369 0.007733054 0.013777693 0.004786741 0.008866448 0.007862236 0.011317762 0.009536368 0.007613333 HiFi - Nmax- 4MM G G A A A T T A G G T G C G C T T G G C A 0.714614956 0.606115446 0.755799511 0.746355936 0.863704127 0.726254107 0.72907491 0.614567346 0.465770598 0.537484475 0.474327411 0.346519393 0.549121492 0.517258765 0.44685551 G 0.778764599 0.684804424 0.668591533 0.792056269 0.828617553 0.628418273 0.845945415 0.607559129 0.667244624 0.667420251 0.755374797 0.597188022 0.594822243 0.317586865 0.511635916 0.536540003 0.448852349 C 0.724593862 0.726226872 0.84765479 0.70623923 0.751512488 0.58152313 0.678906906 0.278919063 0.21753795 0.336782256 0.275058514 0.429854989 T 0.677322639 0.750718332 1 0.807798852 0.93122441 0.840675663 0.654006394 0.796681683 0.52055269 0.745713283 0.340444286 0.547491292 0.527162707 0.685480026 0.593782063 0.51428755 HiFi - ΔRA - 5MM G G A A A T T A G G T G C G C T T G G C A 0.013488138 0.013711889 0.013391259 0.013093427 0.013342359 0.010441711 0.00801563 0.005866916 0.004961434 0.010518112 0.006460232 0.00395747 0.006903403 0.006242252 0.006160751 G 0.013787011 0.013171611 0.011835776 0.012481716 0.014815566 0.012455803 0.01012127 0.006966898 0.006500871 0.008380616 0.011881238 0.007848868 0.008730202 0.004799795 0.009878545 0.008006333 0.005543286 C 0.015143716 0.014561157 0.017012079 0.011880913 0.010647712 0.006675104 0.008030548 0.00329328 0.002616567 0.003602078 0.002817067 0.004673467 T 0.015576995 0.013044364 0.016016283 0.013259044 0.011067532 0.0094376 0.01206167 0.01508101 0.006816867 0.011687607 0.004223877 0.007948571 0.006554022 0.010719955 0.007776679 0.006016019 HiFi - Nmax- 5MM G G A A A T T A G G T G C G C T T G G C A 0.903391971 0.865238013 0.869111872 0.860577111 0.908953194 0.697494149 0.63551422 0.497606511 0.41841042 0.633216596 0.507006926 0.365097958 0.546421872 0.530831992 0.513834077 G 0.858808125 0.831245925 0.777734086 0.821115368 0.942616288 0.791889306 0.778958642 0.56696308 0.558569785 0.587617077 0.675226903 0.539211352 0.607524614 0.373616855 0.590524995 0.561833112 0.496636725 C 0.938282213 0.846241212 0.909353471 0.773784412 0.702674361 0.514305721 0.554379306 0.282270227 0.269831715 0.37390821 0.327519851 0.450214786 T 0.985772411 0.902884942 1 0.902902094 0.852154708 0.776725505 0.770386849 0.902686631 0.479625008 0.787171457 0.366148161 0.542562346 0.522854227 0.798620203 0.608461648 0.522246394 HiFi - ΔRA - 6MM G G A A A T T A G G T G C G C T T G G C A 0.009805116 0.012715501 0.012630048 0.010471479 0.011344192 0.011263502 0.01021833 0.005124976 0.004406585 0.009269639 0.0069353 0.003970228 0.007953171 0.006534229 0.005291394 G 0.012679987 0.011810117 0.011169015 0.011323994 0.011247274 0.010736707 0.006657872 0.006407182 0.002700622 0.005345071 0.006856219 0.006535251 0.0069632 0.00454542 0.009009418 0.006869993 0.00541416 C 0.011847912 0.012137095 0.012325454 0.010922202 0.007772101 0.005228534 0.005865151 0.002910405 0.003153095 0.003012321 0.00476487 0.001688368 T 0.012649779 0.009982743 0.01183054 0.011226513 0.008014786 0.008271993 0.013516912 0.01366025 0.005575577 0.009817178 0.005357081 0.008131427 0.00789824 0.00959958 0.007125887 0.00621139 HiFi - Nmax- 6MM G G A A A T T A G G T G C G C T T G G C A 0.887152797 0.968204258 0.855339948 0.863845549 0.880666285 0.802167793 0.769650166 0.568090896 0.444124197 0.674565791 0.530432544 0.442318126 0.594686652 0.612595177 0.562374729 G 0.869163016 0.773797014 0.880597865 0.852955154 0.85236507 0.689157502 0.71686875 0.615758605 0.499038463 0.584747631 0.654734864 0.508875663 0.635302401 0.420252122 0.600849339 0.578864889 0.531045939 C 0.863508237 0.800096141 0.80582539 0.750287868 0.660067357 0.566057485 0.569130355 0.380296834 0.370090583 0.456489464 0.427197946 0.485680349 T 1 0.93686944 0.976146744 0.929205544 0.794124489 0.770006718 0.842193486 0.905304867 0.543889997 0.75697057 0.513841235 0.652125866 0.537417939 0.801301771 0.589234575 0.545599662 ************************************************** ************************************************** ************************************************** pLibrary EMX1 - nicked pool ΔRA and Nmax for target sequences with different misamtches (MM) (1 to 6 MM) across the target > SpCas9 Sp - ΔRA - 1MM G G C G C G G G C C G C T C G C T C T A A -0.007759234 -0.004475916 -0.006028718 -0.007067363 -0.008041893 -0.012393209 -0.016129014 -0.019131723 -0.01326007 -0.013278239 -0.024617316 -0.026445765 -0.014179213 -0.016234058 -0.024550264 -0.019125643 -0.016754647 G -0.008429344 -0.006455381 -0.00356833 -0.020863721 -0.015351102 -0.049135725 -0.013599638 -0.018055974 -0.01440873 -0.029152966 -0.015750912 -0.011122844 C -0.004312298 -0.013227955 -0.027826991 -0.022294682 -0.013971308 -0.01101983 -0.003723469 -0.014837502 -0.006784842 -0.000144531 -0.005255672 -0.026954697 T -0.007534209 -0.006062998 -0.004820864 -0.006167322 -0.007484734 -0.010925778 -0.044402054 -0.010499711 -0.016557514 -0.013937998 -0.011496486 -0.023081813 -0.017124197 -0.016541285 -0.023389137 -0.024332223 -0.014712643 -0.030405025 -0.019651062 Sp - Nmax- 1MM G G C G C G G G C C G C T C G C T C T A A 0.220333864 0.161610138 0.168879396 0.216848735 0.218783749 0.300079671 0.389391057 0.417704523 0.280259559 0.293286648 0.530215254 0.591410706 0.772203308 0.819802808 0.695569114 0.948793329 0.627489487 G 0.228565628 0.184223995 0.165113827 0.509269321 0.592041157 1 0.305414949 0.378567567 0.77152397 0.685062752 0.843734055 0.742131593 C 0.173897358 0.324249513 0.774616836 0.764673661 0.739358828 0.787845946 0.808455538 0.748323205 0.62295227 0.699345823 0.875307712 0.864510897 T 0.240499471 0.16200651 0.180004359 0.187884057 0.210172822 0.294871241 0.84165553 0.26465992 0.368071133 0.333094097 0.274047853 0.555496449 0.449310661 0.761448965 0.531747284 0.544742255 0.643121974 0.905060063 0.839365117 Sp - ΔRA - 2MM G G C G C G G G C C G C T C G C T C T A A -0.012800359 -0.011946882 -0.011985147 -0.012554144 -0.012978011 -0.012300397 -0.016020358 -0.013126475 -0.013889358 -0.011349349 -0.010477509 -0.010642328 -0.006682004 -0.004854454 -0.012561798 -0.006799688 -0.009933126 G -0.010322115 -0.013225168 -0.016810093 -0.010554711 -0.010236828 -0.01821059 -0.012309846 -0.014551391 -0.00839665 -0.013316287 -0.00750955 -0.003225837 C -0.012553178 -0.014666084 -0.013954426 -0.012378704 -0.006121854 -0.003720924 -0.000994756 -0.004460071 -0.002627549 0.002065458 0.001059831 -0.007760054 T -0.014224711 -0.01266069 -0.013761215 -0.012807753 -0.01595208 -0.014962504 -0.027638822 -0.012404735 -0.014290203 -0.014080629 -0.011351854 -0.011764801 -0.012279065 -0.005296768 -0.013645169 -0.011335344 -0.005704047 -0.010245678 -0.008650554 Sp - Nmax- 2MM G G C G C G G G C C G C T C G C T C T A A 0.453414336 0.476545209 0.451593537 0.523214098 0.606139787 0.61077898 0.709424131 0.633487141 0.64755483 0.608798478 0.639526821 0.767514404 0.726145046 0.775639974 0.735426044 0.767998651 0.729816519 G 0.43617155 0.527015847 0.617462556 0.70135976 0.820033097 0.865231937 0.681691616 0.601617228 0.772222819 0.730967197 0.742169839 0.853424766 C 0.502747659 0.640218159 0.790192733 0.822447687 0.766266915 0.835561824 1 0.872004979 0.989071518 0.972177549 0.910570966 0.723074264 T 0.538657528 0.477391193 0.533202635 0.514046334 0.655417315 0.566385481 0.838944803 0.504915933 0.666082788 0.675400584 0.515370949 0.707461334 0.668873724 0.734800734 0.677804761 0.652147382 0.694997069 0.754457777 0.723860017 Sp - ΔRA - 3MM G G C G C G G G C C G C T C G C T C T A A -0.007553274 -0.005500101 -0.007472839 -0.004845844 -0.00602453 -0.007218229 -0.006022011 -0.007242457 -0.005809315 -0.005125632 -0.005885193 -0.004755496 -0.00187684 -0.002421391 -0.003195313 -0.001214911 -0.003691197 G -0.004983873 -0.005905616 -0.004575088 -0.003806099 -0.005317389 -0.007762718 -0.004953069 -0.00448225 -0.002820749 -0.003865585 -0.00122597 0.000290965 C -0.007754481 -0.005901319 -0.005491844 -0.002532346 -0.001551876 -0.00081411 0.002386973 -0.000873993 0.000811323 0.002053718 0.001759986 -0.002838275 T -0.008845406 -0.007561692 -0.006727934 -0.008213202 -0.005769868 -0.010166855 -0.009284711 -0.007187708 -0.006654564 -0.008238934 -0.008582822 -0.003288232 -0.004078506 -0.000759634 -0.005180809 -0.004697552 -0.004204355 -0.001440931 -0.003389021 Sp - Nmax- 3MM G G C G C G G G C C G C T C G C T C T A A 0.517996623 0.546024557 0.54557316 0.597801016 0.62884184 0.617312236 0.657384939 0.688079292 0.585058201 0.563443614 0.612607025 0.712303863 0.798456257 0.859702177 0.661558329 0.855225393 0.726572462 G 0.556896879 0.557277185 0.629413033 0.621118888 0.621051497 0.657312766 0.633660801 0.533987308 0.815046994 0.708041081 0.864176375 0.845144229 C 0.620052314 0.660210796 0.689884863 0.781844798 0.853042944 0.8573915 1 0.929148239 0.955443003 0.873679284 0.976014959 0.781142389 T 0.65035081 0.540260219 0.59183544 0.577350444 0.620579404 0.598413967 0.632792773 0.569800936 0.577938333 0.701514176 0.567427185 0.655609529 0.545890559 0.81127086 0.598376655 0.632660317 0.743344179 0.743196386 0.844904662 Sp - ΔRA - 4MM G G C G C G G G C C G C T C G C T C T A A -0.00258918 0.000265515 -0.002000573 0.001847462 -0.000187481 0.001374425 0.001651255 0.002336388 -0.000597039 -0.000426716 0.000231282 -0.000394496 -0.000105128 0.00060202 0.001874117 0.002646661 -0.000792568 G -0.001862936 -0.000925302 0.002278831 0.002391147 0.00296897 0.000490638 0.000367946 -0.000297791 0.002226201 -0.000269854 0.001709947 -0.000423506 C 0.000258144 0.002659157 0.000149358 0.003084009 0.002157153 0.002219768 0.001944105 0.002512953 0.002271545 0.002842996 0.001119211 0.000375089 T -0.003314615 -0.002439524 0.000384638 -0.001649187 0.000890678 -0.001974348 -0.000294433 -0.002159995 -0.002202381 0.002015277 -0.001934485 -0.000702517 -0.001305834 0.00129402 -0.002944279 -0.00158068 0.001210142 -0.000387882 0.001460298 Sp - Nmax- 4MM G G C G C G G G C C G C T C G C T C T A A 0.607048092 0.736658309 0.730090563 0.858326071 0.794408743 0.789988118 0.898178052 0.946998556 0.712219833 0.745258007 0.756530932 0.809157728 0.898371654 0.887447212 0.901255408 1 0.762355041 G 0.685872459 0.747432594 0.901306813 0.816876755 0.896136631 0.812798616 0.748788519 0.728700739 0.895401486 0.800124465 0.88463686 0.857826954 C 0.817171718 0.933517659 0.78706602 0.96959201 0.898692975 0.881129393 0.946591893 0.85418546 0.900537372 0.947374846 0.93446393 0.848531127 T 0.698392721 0.631404988 0.771607582 0.674848109 0.821209957 0.743886193 0.727229634 0.67648941 0.668112685 0.928938857 0.631679442 0.798190559 0.682077797 0.822969789 0.749758381 0.723962523 0.861488929 0.82338676 0.877817771 Sp - ΔRA - 5MM G G C G C G G G C C G C T C G C T C T A A 0.001519335 0.00260657 0.002395693 0.003304235 0.002987623 0.002355395 0.00309118 0.004605919 0.002577731 0.002451114 0.002100872 0.002162396 0.00145651 0.001569518 0.003212613 0.002242077 0.001928938 G 0.002941196 0.002486728 0.004383144 0.003425494 0.00363327 0.003490953 0.002563282 0.000943179 0.003686891 0.002651422 0.001321369 0.000709139 C 0.002943277 0.004826302 0.003652332 0.003009709 0.001132729 0.001127398 0.001061801 0.002086256 0.002379749 0.002798835 0.001879032 0.001606902 T 0.001907185 0.00233427 0.00331589 0.003414545 0.003578203 0.005058862 0.004303085 0.002603054 0.000972374 0.004491737 0.002491976 0.002490857 0.000145452 0.001202022 0.002245364 0.00054961 0.003590211 0.001176247 0.003108753 Sp - Nmax- 5MM G G C G C G G G C C G C T C G C T C T A A 0.697952919 0.817625785 0.81213874 0.837255595 0.822601838 0.763367724 0.863924631 0.965829754 0.81681502 0.791391585 0.830612967 0.845933606 0.758246159 0.787014204 0.877103221 0.86506658 0.794597769 G 0.816519052 0.801798602 0.96727003 0.92415095 0.895017832 0.889391393 0.858765672 0.743914474 0.929833946 0.807476747 0.772977996 0.805486606 C 0.906108123 1 0.804474127 0.878960673 0.847497685 0.834577103 0.808715555 0.831640274 0.792678664 0.846417954 0.817539715 0.810573069 T 0.802662317 0.806709045 0.846048105 0.88119925 0.844606073 0.876749583 0.829893203 0.816805242 0.719877861 0.952493266 0.801837786 0.830016535 0.660763955 0.761516443 0.8175607 0.781078283 0.91376019 0.778094404 0.922060091 Sp - ΔRA - 6MM G G C G C G G G C C G C T C G C T C T A A 0.001544527 0.001972559 0.002608082 0.002371888 0.002898401 0.00137295 0.001990965 0.00257121 0.002887666 0.002534913 0.001438751 0.001702767 0.001359039 0.001193729 0.003246732 0.001626591 0.001211814 G 0.001937115 0.003199822 0.002783686 0.002027734 0.002546089 0.002265599 0.001198131 0.002760954 0.001641509 0.002446345 0.002945555 0.002145418 C 0.002125151 0.003080374 0.002826778 0.002314095 0.003093584 0.0016799 0.001287309 0.001401543 0.001151343 0.002580811 0.000687717 0.000982422 T 0.002456898 0.003920541 0.00290289 0.002397994 0.002622346 0.003558085 0.003060307 0.002378294 0.000403504 0.002237977 0.002199601 0.001396707 0.000195649 0.000796363 0.001966505 0.001296211 0.002760247 0.002729271 0.001844877 Sp - Nmax- 6MM G G C G C G G G C C G C T C G C T C T A A 0.74975419 0.847148027 0.898564699 0.796028226 0.875217764 0.763101515 0.806025795 0.915112805 0.860821301 0.910075522 0.869186214 0.908128343 0.860390895 0.858216521 0.882539298 0.852947432 0.861623565 G 0.933544007 0.914799611 0.88270717 0.946003889 0.913757106 0.891315535 0.81529964 0.835789446 0.884469368 0.948612967 0.870623707 0.824824588 C 0.961687878 0.923297553 0.884871749 0.88797163 0.83187789 0.866929229 0.911073841 0.911009621 0.844489652 0.814394543 0.877605217 0.861266579 T 1 0.905607299 0.856623972 0.919984908 0.952732494 0.908016892 0.912030105 0.930404602 0.750420962 0.913762668 0.91181767 0.851600626 0.752013648 0.748556408 0.891852398 0.806811925 0.837462317 0.868270596 0.891890281 ===== > SaCas9 Sa - ΔRA - 1MM G G C G C G G G C C G C T C G C T C T A A -0.004385486 -0.004320323 -0.005745192 -0.00338546 -0.000821428 -0.000793582 -0.001137751 -0.006325162 -0.008377872 -0.010620867 -0.0025174 -0.002063339 0.007512794 -9.6681E-05 -0.008568772 G -0.006004047 -0.007480681 -0.004013692 -0.004604665 -0.002738731 -0.00248916 -0.001637628 -0.001621909 -0.009215283 -0.004614541 -0.048693623 -0.012087624 -0.005828022 -0.010226443 -0.003274893 0.008177064 -0.002587549 C -0.006323366 -0.003290895 -0.002258543 -0.00323165 -0.00426982 -0.000667659 -0.007252016 -0.006138858 -0.005646982 -0.005803332 -0.005417918 -0.009421691 T -0.004995254 -0.009366698 -0.00465065 -0.003384127 -0.019984732 -0.041054907 -0.008271074 -0.039263547 -0.011222774 -0.004722036 -0.004358677 -0.004779663 -0.009031289 0.003539032 -0.002117096 -0.003777191 Sa - Nmax- 1MM G G C G C G G G C C G C T C G C T C T A A 0.140931462 0.120404196 0.156857133 0.13414002 0.113402866 0.113992835 0.133274078 0.180322472 0.241769671 0.270167812 0.095354885 0.104126828 0.403057861 0.118211315 0.215369087 G 0.163155566 0.189377474 0.134833052 0.197921385 0.151103059 0.09208712 0.103911029 0.136503277 0.239251869 0.145293734 1 0.316316997 0.168440244 0.263345889 0.134167319 0.455751747 0.171361352 C 0.177128348 0.1255906 0.101834758 0.137471827 0.174127925 0.092754563 0.230036095 0.18787845 0.192185027 0.176145109 0.217909151 0.378304712 T 0.161613083 0.240684048 0.202070092 0.128136728 0.517503348 0.860356854 0.209209217 0.802488039 0.287026335 0.144006864 0.143776754 0.148955606 0.243312987 0.206535854 0.21716176 0.124090554 Sa - ΔRA - 2MM G G C G C G G G C C G C T C G C T C T A A -0.005197676 -0.005493762 -0.006197993 -0.001023394 0.005211964 0.005397266 0.006502754 0.002240005 -0.001567871 -0.008634603 -0.002266608 0.000881697 0.00474671 0.002791405 -0.013740878 G -0.003320665 -0.009632095 -0.001787291 0.001168511 0.007726985 -0.008381335 0.001110254 0.005648952 -0.002989051 -0.009083052 -0.01962389 -0.004923887 -0.004244334 -0.0236187 -0.014517585 0.008566866 0.001788795 C -0.007813372 -0.002304401 -0.003820746 0.000827229 0.003483662 0.000869359 0.002383301 -0.00308352 -0.001954732 -0.004772644 -0.000935868 0.009831386 T -0.006059017 -0.010701032 0.001683382 -0.001287362 0.000784628 -0.018603422 -0.006304036 -0.021366144 -0.009654199 -0.012727514 -0.007182717 -0.013463673 -0.005046846 0.007224186 0.005619983 -0.003479755 Sa - Nmax- 2MM G G C G C G G G C C G C T C G C T C T A A 0.265779395 0.269609609 0.306602957 0.252346944 0.317552413 0.41698295 0.404511274 0.36654201 0.34720475 0.38140959 0.210595644 0.201809935 0.668178039 0.22357195 0.48535367 G 0.306566828 0.422654164 0.300080827 0.318449922 0.382307715 0.300171773 0.236368764 0.412208069 0.386735984 0.324596324 1 0.412967278 0.298563004 0.631983745 0.454058105 0.61702525 0.302070436 C 0.376828177 0.257151503 0.259126613 0.231851941 0.336911492 0.257165009 0.367565702 0.307147387 0.344559483 0.332101695 0.489541139 0.650642748 T 0.292055043 0.429808584 0.366586292 0.22602417 0.584827087 0.98299523 0.348028263 0.818493274 0.453595046 0.37532535 0.312073498 0.398782893 0.404923245 0.360883485 0.433979569 0.29418742 Sa - ΔRA - 3MM G G C G C G G G C C G C T C G C T C T A A 0.003479419 0.002624638 0.004821226 0.007388871 0.008107798 0.009014244 0.010955928 0.012318964 0.010218563 0.003627967 0.004623016 0.005379253 0.005550752 0.004046601 0.004398254 G 0.005438714 0.003988556 0.006351172 0.008210951 0.009881789 -0.004343974 0.008342853 0.009592335 0.010624834 0.003066402 0.009972746 0.009021568 0.007019618 -0.00853251 -0.012064932 0.00508047 0.006639635 C 0.003564813 0.006478989 0.00605513 0.007622342 0.010813193 0.007081414 0.012503578 0.007707122 0.005206517 0.006477902 0.007984801 0.01821004 T 0.003552775 0.004281175 0.011254489 0.006589482 0.01367664 0.009966868 0.005784818 0.003184665 0.003767845 -0.001912599 0.003045714 -0.003814623 0.00894418 0.005537148 0.009925464 0.006259045 Sa - Nmax- 3MM G G C G C G G G C C G C T C G C T C T A A 0.419870215 0.38266476 0.495472215 0.564669347 0.62860625 0.671614907 0.781237505 0.702466338 0.613458609 0.39845548 0.372284124 0.435062979 0.476004906 0.314100068 0.541635124 G 0.572837422 0.558185434 0.584627957 0.615800762 0.695445445 0.383084193 0.553764313 0.732033545 0.711296757 0.38490331 0.833279497 0.570855154 0.462518236 0.583062021 0.572651538 0.479963543 0.350014804 C 0.543750913 0.530781221 0.528452056 0.635002025 0.805296786 0.55321228 0.756018073 0.558997218 0.42523808 0.399011905 0.47508848 1 T 0.371258217 0.509962809 0.653038455 0.525150952 0.785184327 0.814370718 0.493925654 0.628282879 0.450905034 0.383187156 0.367577886 0.418897453 0.496033931 0.375398843 0.498843209 0.471648026 Sa - ΔRA - 4MM G G C G C G G G C C G C T C G C T C T A A 0.008561963 0.007110173 0.008286171 0.008403118 0.006874613 0.007159504 0.008688591 0.009372506 0.008441407 0.008937051 0.006223892 0.004762593 0.00305966 0.004591342 0.011796721 G 0.009295006 0.009257417 0.008741856 0.009335325 0.008620503 0.005576867 0.010712198 0.00773517 0.012142704 0.008274296 0.016599215 0.012444584 0.009163696 0.009587864 0.003560719 0.00157417 0.005115689 C 0.009847012 0.009604894 0.010496037 0.008570369 0.010605813 0.007934319 0.012984597 0.006844805 0.005227777 0.010276428 0.005736278 0.01092339 T 0.009301133 0.012736483 0.012313766 0.009514238 0.015948557 0.01761747 0.010152274 0.013379706 0.010184797 0.009460534 0.008284316 0.009248562 0.013080253 0.003557202 0.010833256 0.00779692 Sa - Nmax- 4MM G G C G C G G G C C G C T C G C T C T A A 0.674926729 0.512593827 0.63390739 0.694499383 0.573651103 0.576423708 0.657252749 0.623873979 0.559020989 0.643182625 0.49803564 0.468347866 0.334376719 0.440819559 0.736298362 G 0.745076342 0.701647046 0.628061138 0.693026888 0.680030498 0.582163391 0.825694017 0.611696227 0.803629348 0.521829376 1 0.79363842 0.676835224 0.641799967 0.58720845 0.260177715 0.436845954 C 0.777079109 0.70641587 0.774276529 0.755270954 0.846325891 0.677597785 0.831534795 0.558534465 0.443329417 0.62534767 0.478660533 0.719042965 T 0.639605544 0.818035115 0.913477292 0.728131486 0.938747815 0.991698186 0.628801064 0.8192271 0.788311333 0.71490205 0.542016624 0.624242 0.72026954 0.318261497 0.69324836 0.559915837 Sa - ΔRA - 5MM G G C G C G G G C C G C T C G C T C T A A 0.006205034 0.004312938 0.00573035 0.004240534 0.003839303 0.004337233 0.003512736 0.00311639 0.002939097 0.005879298 0.003354566 0.002711671 0.000317703 0.002366901 0.006345182 G 0.005804451 0.005805828 0.004885581 0.003797863 0.004758026 0.007300368 0.005275601 0.002990968 0.005491612 0.004455062 0.008026046 0.00601157 0.004422904 0.009311489 0.008426451 0.000480762 0.003265692 C 0.0078069 0.005833101 0.006562645 0.004230574 0.00418656 0.003920961 0.00594105 0.002425789 0.001897354 0.004192524 0.002281324 0.003153497 T 0.007506035 0.01002968 0.00787857 0.005571302 0.008445967 0.009013211 0.005827491 0.009827383 0.006604378 0.006449481 0.003456222 0.007957095 0.006533701 0.001011168 0.00565806 0.003269074 Sa - Nmax- 5MM G G C G C G G G C C G C T C G C T C T A A 0.492263022 0.35795103 0.422412333 0.399115421 0.362995294 0.352095085 0.340985767 0.28320403 0.269658215 0.440402429 0.2929179 0.259536257 0.592467235 0.263880809 0.426797786 G 0.499892176 0.466312384 0.417565207 0.914208634 0.425453639 0.544044902 0.46560868 0.341185254 0.427809539 0.32876376 0.527435666 0.471315337 0.375709949 0.549956079 0.498330616 0.130380222 0.304529919 C 0.53887926 0.472365051 0.507950097 0.967237693 1 0.367791295 0.44214314 0.287242247 0.228250993 0.361704457 0.285471861 0.31900923 T 0.540228238 0.656535489 0.59649956 0.528923776 0.592920875 0.594503529 0.431961073 0.592524971 0.473227314 0.975123358 0.320658371 0.508543825 0.467320737 0.197781996 0.413009335 0.3121326 Sa - ΔRA - 6MM G G C G C G G G C C G C T C G C T C T A A 0.008122971 0.005596831 0.004970749 0.0055641 0.004982218 0.005149849 0.005694563 0.002560116 0.001679557 0.006511549 0.00153379 0.001410664 0.001395369 0.002285877 0.005098987 G 0.007680689 0.00479572 0.005608428 0.00720015 0.00719092 0.007302686 0.006282171 0.001158776 0.003173195 0.003779971 0.00752134 0.003514452 0.003608383 0.011626496 0.011311692 0.000140671 0.001787061 C 0.00967296 0.006771011 0.006081988 0.005544424 0.004727348 0.005116213 0.005110285 0.001282884 0.002616396 0.002774568 0.001605999 0.000565817 T 0.007901398 0.010533309 0.00977779 0.006661337 0.007994109 0.009220607 0.006387362 0.011816069 0.00534383 0.008459038 0.003943441 0.00625351 0.004859394 0.001034328 0.005388458 0.002416174 Sa - Nmax- 6MM G G C G C G G G C C G C T C G C T C T A A 0.814385632 0.553286996 0.629551459 0.614426635 0.555005242 0.543547029 0.569977381 0.438052321 0.40053762 0.645113456 0.383547972 0.395597841 0.366584285 0.419697945 0.599992261 G 0.65638308 0.693597919 0.617267033 0.774522407 0.612285483 0.726878813 0.66043237 0.514738114 0.488973856 0.468932851 0.69982877 0.54914631 0.544792111 0.738571415 0.744491669 0.270360251 0.37962357 C 0.852385731 0.680253176 0.712240497 0.80480552 0.713489483 0.602862512 0.614331399 0.381042835 0.355984463 0.496697939 0.433740418 0.374277477 T 0.859192853 1 0.861192388 0.722916419 0.822986108 0.817045375 0.63739331 0.940264716 0.65915971 0.907359165 0.455348885 0.644249395 0.594350455 0.30390324 0.538683786 0.412803675 ===== > SpCas9 HF1 HF1 - ΔRA - 1MM G G C G C G G G C C G C T C G C T C T A A -0.000137901 -0.00589297 -0.006116688 -0.019124225 -0.002068233 -0.013605499 -0.034329849 -0.013218515 -0.011477694 -0.009735148 -0.010503034 -0.007375309 -0.009729159 -0.010494438 -0.015131316 -0.016290506 -0.014468963 G -0.002427279 -0.003217226 -0.007853806 -0.010373655 -0.001653955 -0.017866585 -0.006188295 -0.007484304 -0.001284355 -0.011180771 -0.012238857 -0.01007404 C -0.007442314 -0.013804137 -0.015443307 -0.004866849 -0.011999836 -0.004492845 0.000100086 -0.007436116 0.001306753 -0.002079842 -0.007063675 -0.009221248 T -0.005402457 -0.004992357 -0.006469674 -0.003922759 -0.002115438 -0.007404563 -0.009348598 -0.00593848 -0.012284546 -0.0293604 0.00165492 -0.009886091 -0.008717189 -0.004820437 -0.009999345 -0.011128846 -0.010607132 -0.011110462 -0.008614471 HF1 - Nmax- 1MM G G C G C G G G C C G C T C G C T C T A A 0.275657267 0.310808381 0.283218969 0.603799401 0.325372009 0.662891924 1 0.520885226 0.348432704 0.343656527 0.345930441 0.353229624 0.616018812 0.680630235 0.525028305 0.705403877 0.542663315 G 0.423657732 0.279163612 0.376716735 0.565534843 0.514933585 0.535425872 0.238498759 0.294459593 0.577323826 0.541938014 0.619933537 0.63030647 C 0.380101328 0.611882812 0.803061548 0.667366095 0.699851395 0.662269374 0.675821489 0.642580497 0.654078365 0.663735879 0.786006944 0.681460281 T 0.28185579 0.290682751 0.348747596 0.303219346 0.387649558 0.387037351 0.425795772 0.327619028 0.451471717 0.815122669 0.322914672 0.392241973 0.343931228 0.624067069 0.32752498 0.39212532 0.635761489 0.475468675 0.550207805 HF1 - ΔRA - 2MM G G C G C G G G C C G C T C G C T C T A A -0.007945866 -0.00263245 -0.003502911 0.003381832 -0.000230683 0.010671843 0.009908367 0.002932319 -0.004640144 -0.004731497 -0.00643194 -0.005612809 -0.002344045 0.001807912 -0.002277358 -0.000939949 -0.006756154 G -0.003481428 -0.002306977 0.005845724 0.006357551 0.003138261 -0.006988221 -0.005364092 -0.002159453 -0.001300517 -0.006433459 -0.003280309 -0.00512002 C 0.002168422 0.00557015 0.001474997 0.007401051 0.001762402 0.002114382 0.000401614 -0.005659146 -0.003057092 -0.003410676 -0.001649086 -0.008832235 T -0.008392169 -0.005556179 -0.002327733 -0.007394267 0.00115751 -0.007426004 -0.008214036 -0.00710114 -0.004221603 0.006309055 -0.008287685 -0.008188782 -0.004526311 -0.00344698 -0.007190155 -0.00903558 -0.010302918 -0.004397235 -0.003429916 HF1 - Nmax- 2MM G G C G C G G G C C G C T C G C T C T A A 0.475216089 0.505283605 0.484526147 0.665281262 0.648125073 0.760415296 0.995491764 0.753345989 0.524098958 0.633367827 0.497494043 0.619078808 0.481863803 0.678126713 0.655659005 0.660842146 0.492017223 G 0.522985157 0.53163013 0.721323261 0.775062622 0.758159606 0.71103812 0.525850959 0.443116473 0.622297424 0.586485215 0.494979266 0.563774879 C 0.670861625 0.708202937 0.671922164 0.838447465 0.640538863 0.637076797 0.491186031 0.596447677 0.786451309 0.678459006 0.483603338 0.551263903 T 0.414760342 0.497666843 0.57624036 0.567534244 0.758474944 0.68509757 0.706286461 0.509535889 0.533333111 1 0.441324953 0.604076063 0.528632106 0.523395375 0.551686274 0.567545526 0.823048669 0.476713364 0.51733547 HF1 - ΔRA - 3MM G G C G C G G G C C G C T C G C T C T A A 0.006768774 0.009119682 0.007766671 0.013003828 0.01494987 0.009446198 0.019924706 0.018371993 0.006725467 0.015751046 0.005415255 0.005912662 0.003819157 0.007972826 0.007956347 0.009679386 -0.00172859 G 0.009564943 0.010753491 0.014289979 0.013672122 0.012042096 0.015799836 0.001284433 0.002939185 0.005769488 0.005601025 0.000418518 0.001209071 C 0.014742256 0.012893127 0.016680452 0.015493919 0.007165196 0.00677492 0.001902748 0.005112348 0.012836817 -0.000273232 0.004716806 0.002388065 T -0.00028681 0.005078209 0.013546322 0.009769378 0.016376874 0.01638137 0.016816944 0.011721611 0.005468427 0.023224124 0.001826514 0.009237413 0.006224816 0.003793663 0.005158389 0.003635787 0.016364649 0.001197226 0.005439928 HF1 - Nmax- 3MM G G C G C G G G C C G C T C G C T C T A A 0.624479307 0.695602272 0.749539842 0.811807915 0.824328285 0.752483891 0.971063806 0.941692142 0.710201721 0.871822977 0.637752188 0.678223923 0.577958298 0.720341216 0.678622089 0.76272961 0.571999934 G 0.74917713 0.749244264 0.89776952 0.874129242 0.801302713 0.846588784 0.630451093 0.573414412 0.632947099 0.874050127 0.574810181 0.545718813 C 0.858881591 0.936922347 0.88904633 0.867051129 0.700561375 0.625900598 0.557043908 0.642649639 0.769898655 0.652718272 0.551046928 0.630337192 T 0.648123967 0.631104333 0.856995381 0.783090887 0.886884995 0.865895385 0.891369932 0.794221609 0.744524882 1 0.666444849 0.715352133 0.612715119 0.578643285 0.686017059 0.723824359 0.98210109 0.51816915 0.672613056 HF1 - ΔRA - 4MM G G C G C G G G C C G C T C G C T C T A A 0.009364779 0.010734582 0.014797062 0.009925355 0.012322351 0.006994509 0.012783352 0.013048505 0.012578568 0.013380897 0.006384532 0.007366127 0.004953321 0.00364484 0.005475455 0.005218172 0.00291779 G 0.013092901 0.011038339 0.01010083 0.004910968 0.005222534 0.011667684 0.008427756 0.007561824 0.00573824 0.018196665 0.004381763 0.007032734 C 0.012572988 0.011141535 0.012076384 0.008505321 0.004107036 0.001342487 0.00092663 0.002840898 0.004887246 0.000716249 0.001280492 0.009093264 T 0.011983458 0.011506808 0.015061677 0.012644684 0.014417685 0.016718388 0.014939967 0.013058635 0.014255193 0.013768489 0.011029876 0.00790801 0.00897815 0.004739455 0.011024039 0.011525094 0.014161214 0.003385019 0.003990491 HF1 - Nmax- 4MM G G C G C G G G C C G C T C G C T C T A A 0.720082386 0.799115575 0.880600538 0.861049521 0.87176692 0.733748034 0.922748831 0.943008628 0.765537456 0.920550491 0.696899237 0.750269544 0.647542998 0.653884726 0.75360464 0.696565865 0.615093998 G 0.824102233 0.799454408 0.871357605 0.72586117 0.756746579 0.819366065 0.695234441 0.669908747 0.657859701 0.865514233 0.635523142 0.677602401 C 0.896935898 0.896298984 0.838834886 0.731920295 0.611528939 0.618826972 0.51295291 0.592221299 0.643943992 0.567966063 0.55725516 0.706311955 T 0.773792782 0.75248841 0.87780691 0.808875181 0.93244973 0.943787947 0.935764887 0.893978654 0.776490278 1 0.752603678 0.75781547 0.672130161 0.637191076 0.780996867 0.709685795 0.905988268 0.570895135 0.645383996 HF1 - ΔRA - 5MM G G C G C G G G C C G C T C G C T C T A A 0.009439623 0.008865937 0.006462648 0.007960964 0.006579201 0.005359695 0.003879962 0.007741833 0.00803559 0.007440645 0.002394043 0.003353341 0.000453886 0.000835825 0.003935654 0.001926546 0.003277251 G 0.00841068 0.007725115 0.00472465 0.004444714 0.00366949 0.003868273 0.006556124 0.006226022 0.001702677 0.010576763 0.002502763 0.005479006 C 0.010951574 0.007971211 0.005259274 0.001393556 0.001704233 0.001700153 -0.00138045 0.003168277 0.001076602 0.00028745 -0.001016874 0.005659982 T 0.01014877 0.009112753 0.009625938 0.010280595 0.00807697 0.010871047 0.007381831 0.0107394 0.008624741 0.006259848 0.011122094 0.005366012 0.005507925 0.001736046 0.008759033 0.008179527 0.00923496 0.001697975 0.003872601 HF1 - Nmax- 5MM G G C G C G G G C C G C T C G C T C T A A 0.824904162 0.893014591 0.8670353 0.821531563 0.826513317 0.723036371 0.793103583 0.874612612 0.815852054 0.828686748 0.764449548 0.798036202 0.659377905 0.657116557 0.755110974 0.685097492 0.803745606 G 0.904902894 0.883220963 0.928695505 0.788443759 0.75176689 0.831798596 0.811545827 0.870473282 0.792080836 0.901791926 0.695982455 0.733951601 C 0.972795753 0.923695073 0.784333588 0.690685835 0.690310554 0.673239688 0.622856761 0.669163807 0.68246401 0.674221142 0.628063176 0.795940244 T 0.921850307 0.908603075 0.89997767 1 0.840796675 0.970941802 0.877718432 0.886380449 0.809005568 0.878178837 0.990210742 0.780788062 0.719927602 0.678161332 0.899166065 0.816819109 0.875966692 0.656673407 0.789476249 HF1 - ΔRA - 6MM G G C G C G G G C C G C T C G C T C T A A 0.005946843 0.003894139 -7.44725E-05 0.004012139 0.002568802 0.006217326 0.003880951 0.001144628 0.001649281 0.001514821 -0.000558577 -0.002933892 -0.002310614 -0.001382665 -0.000719914 -0.002448276 -0.004012975 G 0.002371183 0.000169875 -0.001094876 -0.000518396 0.001129656 0.004465497 0.003436978 0.004647798 0.001394082 0.006097589 -9.49946E-05 0.000650968 C 0.004792337 0.002565382 -0.000242579 0.001046223 -0.002329282 -0.004929882 -0.007377279 -0.001776281 -0.000687657 0.000135261 0.000660331 0.000941111 T 0.007103525 0.005769747 0.003718189 0.004992851 0.002219198 0.002728824 0.002787695 0.005497876 0.005284244 0.001484832 0.002172248 0.000967566 0.004210554 0.002861475 -0.000395772 0.004631484 0.002646355 -0.000184691 -0.000260703 HF1 - Nmax- 6MM G G C G C G G G C C G C T C G C T C T A A 0.863101578 0.88422812 0.905192938 0.811328488 0.891561255 0.844033697 0.860168862 0.864910709 0.810414293 0.857928802 0.846963879 0.854670965 0.771750203 0.741086367 0.826571606 0.756827656 0.885957889 G 1 0.946415027 0.919316016 0.890699934 0.80125474 0.829894715 0.810439002 0.828733763 0.845241854 0.983970763 0.808837348 0.797660398 C 0.937205442 0.85885267 0.826183892 0.779242158 0.760374995 0.791052802 0.814352842 0.837613129 0.810567627 0.758617603 0.798133507 0.836991798 T 0.983411853 0.944330411 0.920404811 0.958434333 0.897147697 0.908299703 0.913921717 0.930832067 0.863161615 0.87177454 0.926549253 0.789035906 0.854847779 0.726488153 0.877603858 0.819775892 0.836923974 0.780007413 0.807592892 ===== > HypaCas9 Hypa - ΔRA - 1MM G G C G C G G G C C G C T C G C T C T A A -0.00294746 -0.007108668 -0.005586698 -0.021732908 -0.014078359 -0.013436128 -0.040254881 -0.033108824 -0.012266831 -0.011581188 -0.013943623 -0.019212272 -0.021533947 -0.021361748 -0.024517632 -0.032331723 -0.021411782 G -0.007076551 -0.007928135 -0.019169119 -0.015133132 -0.011819795 -0.019619715 -0.009494143 -0.007128213 -0.023102182 -0.020306769 -0.030816954 -0.018792416 C -0.007870434 -0.02432489 -0.016619208 -0.014822013 -0.020990134 -0.018048295 -0.006027455 -0.015253603 -0.012307888 -0.001837568 -0.016945303 -0.030353786 T -0.006924088 -0.002739755 -0.013065024 -0.010762303 -0.016086356 -0.015532066 -0.008590966 -0.007939581 -0.017332767 -0.032891459 -0.00793233 -0.015213929 -0.009991992 -0.022096471 -0.006800527 -0.008463299 -0.020662452 -0.02650069 -0.027572941 Hypa - Nmax- 1MM G G C G C G G G C C G C T C G C T C T A A 0.198527154 0.240217748 0.252809503 0.651544124 0.42042874 0.555082951 1 0.82162497 0.310267987 0.293307115 0.360405905 0.42858942 0.714948924 0.794373967 0.552727269 0.930394518 0.558632474 G 0.266884613 0.280187327 0.522192167 0.620187943 0.568787625 0.537587333 0.254944613 0.231005286 0.749495619 0.529673239 0.802676563 0.703227252 C 0.302579079 0.68781533 0.725412734 0.723484963 0.838117366 0.839029013 0.839677665 0.796977603 0.724226941 0.802691603 0.864902768 0.68527027 T 0.266337952 0.154033824 0.404149531 0.344454883 0.487104003 0.420509034 0.314486993 0.30919682 0.428636418 0.701042154 0.290926419 0.384232347 0.295618877 0.838033967 0.254282298 0.262798704 0.651808673 0.635011811 0.718341311 Hypa - ΔRA - 2MM G G C G C G G G C C G C T C G C T C T A A -0.012144722 -0.011098987 -0.011854543 -0.005299206 -0.00989177 -0.003180356 -0.00468013 -0.00461946 -0.01288182 -0.010956475 -0.01367484 -0.012859473 -0.011044853 -0.008229935 -0.010850057 -0.007513413 -0.012716794 G -0.012671441 -0.011285522 -0.008995639 -0.004720778 -0.005815111 -0.009596106 -0.014947365 -0.013060263 -0.010279165 -0.013704422 -0.00932665 -0.00932598 C -0.010014646 -0.004704119 -0.006562155 8.71751E-05 -0.006636661 -0.008209844 -0.002922459 -0.008604997 -0.010142355 -3.01886E-05 -0.004384609 -0.014829828 T -0.016166897 -0.014864443 -0.010575289 -0.017236407 -0.010258814 -0.018323273 -0.00920999 -0.014672834 -0.012173603 -0.010763117 -0.014136429 -0.015922745 -0.011406848 -0.008620992 -0.015344583 -0.017333014 -0.017944765 -0.009578245 -0.009155737 Hypa - Nmax- 2MM G G C G C G G G C C G C T C G C T C T A A 0.45862878 0.517929675 0.571074235 0.714735195 0.695598302 0.735332366 0.916694677 0.81007682 0.610323823 0.589031293 0.624402938 0.736586207 0.661033259 0.707744857 0.711864663 0.760651887 0.610358993 G 0.463249011 0.530563869 0.771137547 0.833583379 0.803094796 0.777389845 0.694699138 0.531186583 0.68408318 0.659137132 0.61531438 0.68979681 C 0.643932654 0.75717403 0.659845343 1 0.704873476 0.715276739 0.698367993 0.711984066 0.839190751 0.725923655 0.588225142 0.646036356 T 0.506408333 0.495995265 0.681880907 0.666127356 0.761505599 0.753558243 0.657183341 0.593764852 0.624225866 0.872055239 0.506374897 0.739108909 0.606699512 0.657442343 0.624326132 0.653002237 0.888363658 0.600918641 0.632313301 Hypa - ΔRA - 3MM G G C G C G G G C C G C T C G C T C T A A -0.004703383 -0.002030859 -0.000984917 0.00311607 0.000833447 0.001636304 0.00490254 0.006241461 -0.003143147 -0.002571914 -0.002323245 -0.002241848 -0.002282333 -0.000649534 -0.000709323 0.001436303 -0.004216201 G -0.0034115 -0.003115008 0.004828983 0.00450887 0.004061926 0.00446663 -0.001123519 -0.003760244 -0.001050634 -0.006423202 -0.003207707 -0.003326476 C 0.000567744 0.005447012 0.000844319 0.008455619 0.000246026 0.000951541 -0.000841615 -0.001559292 0.000787966 0.002701668 5.12183E-05 -0.004229076 T -0.008577702 -0.006021042 0.001365808 -0.005294992 0.003241866 -0.00046152 0.00091336 -0.002618659 -0.002986149 0.005788269 -0.00644941 -0.001417683 -0.001918736 -0.002527016 -0.004845107 -0.005426843 0.000892592 -0.003180234 -0.000675753 Hypa - Nmax- 3MM G G C G C G G G C C G C T C G C T C T A A 0.462018406 0.593611543 0.672429844 0.856540103 0.802248997 0.721983782 0.95414718 1 0.569186166 0.568847566 0.605152405 0.688542548 0.552391639 0.715291395 0.689985433 0.834772387 0.535574712 G 0.540211339 0.579488013 0.885597973 0.878407362 0.789544233 0.832146815 0.659047588 0.444078022 0.620193427 0.635136861 0.567417671 0.554173639 C 0.794183224 0.931832 0.80713585 0.976001802 0.716641179 0.642125307 0.602000559 0.659364677 0.764644864 0.696133192 0.62109143 0.531254027 T 0.554808075 0.488321515 0.773768534 0.624628506 0.834537981 0.669148585 0.732923287 0.638806559 0.603790915 0.961326462 0.51792383 0.629693249 0.536311764 0.568724081 0.550195074 0.560425928 0.838032302 0.526485281 0.652990684 Hypa - ΔRA - 4MM G G C G C G G G C C G C T C G C T C T A A -7.24145E-05 0.003258319 0.004186 0.004970799 0.005244094 0.002258326 0.004058499 0.005764819 0.00220157 0.002576847 0.00147262 0.001873161 0.000335492 0.000598528 0.003451355 0.003046776 -0.000227214 G 0.00414588 0.003249798 0.005058464 0.003253014 0.001781136 0.004492966 0.00244168 0.001172961 0.001092013 0.004596006 0.000472396 0.000270379 C 0.005031816 0.004706699 0.004951462 0.003935497 -0.000559565 2.519E-05 -0.001662203 -0.000301581 0.001537633 0.002974697 -0.000266772 0.00056111 T 1.14164E-05 5.41312E-05 0.004491091 0.00266959 0.005456992 0.004676873 0.006114015 0.005154626 0.004283093 0.005275679 0.001905462 0.001827341 0.000906717 -0.001548413 0.00154963 0.001274016 0.006617863 -0.001488195 0.001890329 Hypa - Nmax- 4MM G G C G C G G G C C G C T C G C T C T A A 0.660061338 0.77721331 0.874588931 0.929226583 0.885295805 0.778717356 0.957278954 0.991665426 0.736948436 0.775309267 0.724232656 0.768011796 0.698580067 0.727392786 0.865531623 0.83646653 0.660897825 G 0.72029112 0.784377303 0.926488918 0.798997414 0.761416644 0.8730132 0.755435775 0.649517292 0.696184076 0.776206314 0.671508539 0.657992829 C 0.90308827 0.938854599 0.839734837 0.828982008 0.681795372 0.605304593 0.598868217 0.645255276 0.694859469 0.688711274 0.628040823 0.69813138 T 0.715066461 0.674513111 0.870320195 0.809012328 0.888250361 0.915918685 0.924430506 0.859821249 0.78510162 1 0.701597699 0.727462232 0.631856925 0.631155136 0.766263314 0.69745759 0.940506678 0.607899536 0.736571142 Hypa - ΔRA - 5MM G G C G C G G G C C G C T C G C T C T A A 0.002587008 0.003116057 0.004305419 0.003626508 0.002648185 0.000926854 0.000126847 0.002337317 0.001296657 0.001220245 -0.000393929 0.000922995 -0.001179102 -0.001023407 0.00132199 -0.000382813 0.001712746 G 0.003600675 0.002683027 0.003999127 0.00219999 0.000857379 0.001838102 0.000141679 0.001552634 1.03262E-05 0.004235245 0.000212636 0.000197652 C 0.006309924 0.004460612 0.002200045 -0.000145326 -0.00145214 -0.00156634 -0.002226518 0.00023802 -0.001580781 0.003318717 -0.000315574 -0.00013747 T 0.002533629 0.002502112 0.003773343 0.003585821 0.003261931 0.003684556 0.004130298 0.003718341 0.002835027 0.002872955 0.004759893 -0.000127049 0.001093248 0.000733263 0.004216558 0.001097124 0.004023741 -0.00077118 0.001252439 Hypa - Nmax- 5MM G G C G C G G G C C G C T C G C T C T A A 0.793925394 0.861718353 0.871469373 0.866345325 0.830023928 0.753475216 0.786306319 0.884050483 0.824494109 0.764857197 0.795577109 0.824294644 0.679899212 0.711193867 0.864835436 0.828256619 0.780790637 G 0.855757786 0.85665769 0.96736092 0.816573891 0.79318745 0.891750953 0.82235172 0.768303696 0.800705209 0.837019055 0.664183505 0.725839627 C 0.97966755 0.998262825 0.820881614 0.718248171 0.688961652 0.672927974 0.619228667 0.65794639 0.683361148 0.697486303 0.644207776 0.795912752 T 0.883653781 0.902696274 0.871119703 0.948537259 0.872924151 1 0.918385999 0.904855539 0.765733077 0.933168201 0.925026317 0.750753683 0.676529455 0.679576924 0.881591306 0.7893255 0.954302133 0.69990588 0.824545044 Hypa - ΔRA - 6MM G G C G C G G G C C G C T C G C T C T A A 9.73211E-05 0.00077584 0.000741312 -0.000276311 -0.00048697 -8.73862E-05 -0.000484749 6.86876E-05 -0.002419588 -0.002363239 -0.00164111 -0.001311465 -0.001377092 -0.004716924 0.001241789 -0.001054556 0.000757257 G 0.002275115 -0.000770766 0.003302818 -0.000488606 -0.003439508 -0.001501627 -0.003021909 -0.001633369 0.000677218 0.001631955 -0.002273274 -0.002348579 C 0.001244466 0.001579431 -0.002222077 -0.00120223 -0.003272577 -0.001993932 -0.00367849 -0.002163285 -0.001574919 0.00288001 -0.003645263 -0.004118672 T -0.001007712 0.000532323 0.001079145 0.002744535 0.001930679 2.24946E-06 0.001412345 0.000138373 0.001085422 0.001165629 0.003233337 -0.002244158 -0.000482989 -0.003406196 -0.000173604 -0.002342868 0.001362147 -0.0028945 -0.001128332 Hypa - Nmax- 6MM G G C G C G G G C C G C T C G C T C T A A 0.877482796 0.87342648 0.909759522 0.839826132 0.904899225 0.859432141 0.820846415 0.893894721 0.820993027 0.856160462 0.865013142 0.901905475 0.743909649 0.798974066 0.843635492 0.782234384 0.885224781 G 0.933096612 0.903840701 0.918164036 0.850198363 0.838901907 0.903545755 0.786006272 0.805034626 0.847117203 0.92377566 0.80022745 0.761662389 C 0.994071586 0.941501642 0.833065119 0.80053435 0.752211476 0.764628229 0.809058598 0.844779143 0.805127992 0.720673012 0.756349893 0.777663155 T 1 0.935476483 0.910195995 0.950758479 0.977325903 0.914026063 0.922027246 0.980519433 0.825638681 0.862194929 0.927036602 0.803272245 0.808337564 0.718985791 0.928204877 0.795188853 0.8330727 0.800493922 0.830634768 ===== > HiFi Cas9 HiFi - ΔRA - 1MM G G C G C G G G C C G C T C G C T C T A A -0.004278398 -0.002588275 -0.003898978 -0.003224158 -0.003890909 -0.008384855 -0.00960685 -0.0105617 -0.009731362 -0.009067483 -0.016576015 -0.011687582 -0.001428445 0.0015988 -0.008733744 0.001016253 -0.007422228 G -0.003740726 -0.003466145 -0.002323749 -0.0081628 -0.006027048 -0.024706741 -0.010141699 -0.017736227 -0.003236706 -0.014868767 -0.007642608 0.003886944 C -0.001216337 -0.008475349 -0.017745083 -0.002726152 0.001231009 -0.001678148 0.011740521 0.004520122 0.005224475 -0.001010358 0.011331137 -0.009440341 T -0.005077389 -0.002218409 -0.00167764 -0.005247448 -0.005880233 -0.011170141 -0.055829385 -0.005777986 -0.013743533 -0.0079463 -0.007206777 -0.013531269 -0.009311085 0.002812446 -0.014945789 -0.010419664 -0.00034445 -0.018571617 -0.002289122 HiFi - Nmax- 1MM G G C G C G G G C C G C T C G C T C T A A 0.14862292 0.118384609 0.131373249 0.142148496 0.152214702 0.245800471 0.271142206 0.280164207 0.220143003 0.217334925 0.377374702 0.367598872 0.413656304 0.47353698 0.435343811 0.546935699 0.387731377 G 0.138096956 0.13322099 0.118229369 0.280956522 0.377406254 0.5849773 0.238157505 0.358013619 0.450010484 0.44290666 0.526935579 0.439302307 C 0.101372655 0.231552181 0.584279667 0.362934568 0.438138901 0.455995778 0.569225567 0.468018984 0.451643611 0.549432076 0.59270231 0.518219191 T 0.1527755 0.099369021 0.12786056 0.157309514 0.178210142 0.26951718 1 0.187185773 0.303920684 0.243636853 0.206960487 0.353737546 0.296647107 0.474379187 0.350044276 0.327471895 0.362552513 0.651482694 0.452269424 HiFi - ΔRA - 2MM G G C G C G G G C C G C T C G C T C T A A -0.005327999 -0.003744629 -0.004712662 -0.003222171 -0.004249087 -0.003962313 -0.003505003 -0.001521166 -0.002705322 0.001426176 -0.000256166 0.001290167 0.008916711 0.01124667 0.005871102 0.012301283 0.005667572 G -0.006192734 -0.003296646 -0.005034587 0.005722157 0.003791211 -0.000900785 0.000996714 -0.005756466 0.007285177 0.001086894 0.008023439 0.014413133 C -0.002828929 -0.001276195 -0.001418529 0.007398355 0.011914863 0.011959153 0.01607621 0.013261069 0.018748437 -0.000590554 0.015497413 0.004808245 T -0.007909071 -0.005934012 -0.00660717 -0.00659252 -0.001831224 -0.007162428 -0.030381554 -0.002485463 -0.004315233 -0.003405625 -0.004169264 0.002731957 0.001401279 0.009794559 -0.003246559 -0.001739451 0.007501718 0.002921011 0.006623766 HiFi - Nmax- 2MM G G C G C G G G C C G C T C G C T C T A A 0.435882055 0.422990044 0.421714041 0.465061401 0.572347016 0.584612951 0.633008462 0.558462834 0.578801654 0.554487422 0.590941987 0.717412334 0.733573502 0.777148287 0.691145527 0.839675271 0.722278136 G 0.42892416 0.454616963 0.501046664 0.717047866 0.814383216 0.747489392 0.660423348 0.575372544 0.782911214 0.648275009 0.748985023 0.820641761 C 0.450641961 0.575858095 0.769020208 0.785370001 0.770172266 0.840839844 0.829635844 0.852977482 0.936393885 0.836884544 0.809427905 0.72061697 T 0.515003153 0.452558191 0.500069993 0.490055928 0.56432329 0.546229928 1 0.444124806 0.633500048 0.596158793 0.490690873 0.682931488 0.624099016 0.767036971 0.621440569 0.584475875 0.700240167 0.72740719 0.704588261 HiFi - ΔRA - 3MM G G C G C G G G C C G C T C G C T C T A A 0.004430265 0.008990473 0.009123693 0.012539534 0.011510364 0.008691134 0.010596986 0.012691078 0.01056063 0.009398735 0.011460334 0.013353349 0.014932086 0.017551663 0.012541138 0.01643806 0.014291655 G 0.006752279 0.009264017 0.013500567 0.015491099 0.014238195 0.013238143 0.012791314 0.009628279 0.016252879 0.013396277 0.01731262 0.017953479 C 0.010648339 0.016333085 0.012085622 0.018762546 0.016969927 0.01547167 0.019491253 0.015668768 0.017521894 -0.000131739 0.018088128 0.015981651 T 0.006381859 0.0052515 0.008257021 0.005732254 0.011530918 0.005181685 0.005591892 0.007998666 0.009782426 0.011209868 0.005132473 0.012203221 0.009649928 0.01540746 0.012607647 0.010151206 0.013088662 0.013741868 0.017319043 HiFi - Nmax- 3MM G G C G C G G G C C G C T C G C T C T A A 0.669514766 0.740231082 0.743133116 0.844655638 0.839054081 0.805014841 0.898603627 0.926390593 0.78253252 0.741927211 0.8219645 0.887876071 0.847505775 0.978901479 0.823291181 1 0.843743125 G 0.745862602 0.760952611 0.866667442 0.934659288 0.905319793 0.914013461 0.859783721 0.718423766 0.871254379 0.868971106 0.958796555 0.902334134 C 0.830144368 0.975369683 0.935349327 0.99888291 0.914131623 0.87885516 0.935153923 0.934462309 0.938031473 0.892135047 0.927406265 0.851913684 T 0.827765195 0.676989555 0.785759895 0.739238118 0.846106763 0.744013785 0.785337868 0.74960459 0.790656749 0.92807751 0.702954068 0.824673649 0.717530339 0.869190548 0.80398137 0.785313712 0.855638401 0.847439892 0.955322782 HiFi - ΔRA - 4MM G G C G C G G G C C G C T C G C T C T A A 0.011079556 0.013037423 0.013322114 0.017304541 0.016182736 0.01512739 0.016955167 0.019618617 0.012734332 0.013878224 0.012119137 0.012370447 0.013990603 0.013812453 0.01519853 0.017529306 0.011889933 G 0.012464661 0.014694652 0.019082271 0.016536408 0.017291716 0.016759963 0.012972081 0.011937874 0.014454214 0.012839672 0.014131549 0.012323715 C 0.015426645 0.02039119 0.014159201 0.017752916 0.014288254 0.012911394 0.011907117 0.013315037 0.012914225 0.000542879 0.012777179 0.012427953 T 0.011172027 0.011004686 0.016362529 0.011382762 0.015628096 0.014209278 0.01413508 0.011180237 0.011220756 0.017526488 0.011322381 0.013215852 0.009819823 0.013009248 0.012373007 0.010618299 0.015716888 0.012778261 0.014904525 HiFi - Nmax- 4MM G G C G C G G G C C G C T C G C T C T A A 0.638683828 0.736395693 0.74666572 0.873727875 0.793310578 0.819479505 0.928567812 1 0.663202729 0.689099216 0.674860302 0.697684642 0.707384443 0.719684361 0.811737002 0.917724099 0.641711142 G 0.719339097 0.730871713 0.920730145 0.834279192 0.8446967 0.80325021 0.676853151 0.61298309 0.689218619 0.653622217 0.692777025 0.659904506 C 0.828784502 0.97346039 0.763626121 0.887308097 0.701443269 0.652984212 0.653686156 0.648165845 0.678001887 0.730537057 0.666593197 0.658448216 T 0.689833904 0.626021612 0.791024998 0.682711239 0.795699461 0.76353806 0.738160605 0.649451539 0.641155671 0.918186386 0.616062563 0.690788203 0.592535536 0.633308332 0.647509078 0.615981934 0.783920213 0.653708714 0.766487412 HiFi - ΔRA - 5MM G G C G C G G G C C G C T C G C T C T A A 0.013571396 0.014912659 0.016119477 0.015756565 0.01521412 0.014070557 0.016895589 0.019046735 0.015187305 0.014900155 0.012905351 0.014433271 0.010871138 0.012372165 0.015696415 0.016214811 0.013753896 G 0.018083086 0.016523476 0.019320671 0.017282956 0.017089449 0.017404705 0.015705107 0.012290277 0.014997095 0.014219701 0.01337097 0.013128033 C 0.018966803 0.020277634 0.015379364 0.014580497 0.013606007 0.013012095 0.011664733 0.012168184 0.012968012 0.000760681 0.011985344 0.013599097 T 0.016337799 0.016186522 0.016015302 0.017533852 0.017091766 0.018224193 0.017895741 0.01598268 0.013124557 0.01837052 0.016430941 0.014245451 0.011061627 0.011763115 0.014956354 0.013515003 0.016639872 0.012092465 0.015588912 HiFi - Nmax- 5MM G G C G C G G G C C G C T C G C T C T A A 0.746300319 0.810206016 0.792819411 0.845340301 0.794613966 0.77799863 0.86844493 0.957469188 0.76614421 0.748749409 0.741760455 0.734799796 0.631151052 0.687649074 0.816843918 0.838397326 0.711703997 G 0.836950584 0.818252619 0.955023159 0.875945726 0.84882294 0.888846883 0.777991463 0.67232957 0.779960274 0.706658263 0.644560015 0.686530909 C 0.901166346 1 0.786986355 0.771591241 0.677324759 0.653752982 0.606803452 0.66246483 0.642193081 0.696968645 0.651755241 0.655862353 T 0.79219445 0.809676158 0.843561861 0.89953597 0.835891391 0.893833229 0.854570896 0.831800568 0.699275821 0.924940992 0.818551409 0.709860523 0.620969827 0.650709275 0.734942389 0.71878376 0.859923144 0.676398783 0.83688249 HiFi - ΔRA - 6MM G G C G C G G G C C G C T C G C T C T A A 0.013620697 0.013397241 0.015591731 0.014866287 0.016240509 0.013506267 0.013829705 0.016350203 0.016181198 0.015085086 0.013085799 0.013545237 0.012622531 0.013065744 0.015160218 0.012245951 0.014785795 G 0.017394102 0.014458629 0.016818535 0.017412584 0.015639353 0.015165178 0.014424649 0.015179538 0.014549905 0.017401569 0.015479655 0.011874687 C 0.017461333 0.018675043 0.015821882 0.014416852 0.014358515 0.01471399 0.012938867 0.015488897 0.013687949 0.000182731 0.013281445 0.012390121 T 0.018936723 0.018519888 0.016726153 0.016207248 0.017659749 0.017124079 0.016748413 0.018746423 0.013050444 0.015173832 0.017627771 0.014222426 0.012280195 0.011028725 0.016850261 0.013036961 0.016882419 0.01280813 0.015711535 HiFi - Nmax- 6MM G G C G C G G G C C G C T C G C T C T A A 0.817293894 0.854659104 0.829636354 0.823532148 0.839721167 0.796766768 0.812553498 0.868627027 0.762646731 0.854190719 0.790985692 0.7958308 0.72174441 0.745801307 0.793550421 0.761266608 0.776509429 G 0.899311017 0.89409286 0.848551081 0.887655069 0.918510719 0.887182641 0.730762831 0.716585484 0.761313449 0.842665632 0.753438291 0.688769571 C 0.932260446 0.914683067 0.872813722 0.789667613 0.713715529 0.717446033 0.736367714 0.777021775 0.718664934 0.682829335 0.722449951 0.70868852 T 1 0.851932293 0.857524077 0.910352254 0.908876576 0.895694883 0.867983871 0.916259825 0.750331527 0.86684333 0.903844152 0.77814718 0.739865713 0.657511214 0.835996535 0.762104255 0.790721684 0.722059305 0.815263512