Sequence determinants of Cas9 cleavage activity for the supercoiled pool The tables below contain the raw data plotted in bubble heatmaps where max Δ abundance defined the gradient colour and abundance-min defined the bubble size. > Sp = SpCas9 Sa = SaCas9 HF1 = SpCas9 HF1 Hypa = HypaCas9 HiFi or IDT = IDT Alt-R HiFi Cas9 > 002 = pLibrary PS4 = KMlib002 003 = pLibrary EMX1 = KMlib003 > MM = mismatch > base = possible MM with crRNA (A, T, G, C) > position = position of MM in the target/crRNA where 1 is the 3' PAM proximal end and 20 is the 5' PAM-distal end ************************************************** Sp 1MM 002 (PS4) Sa 1MM 002 (PS4) HF1 1MM 002 (PS4) Hypa 1MM 002 (PS4) HiFi 1MM 002 (PS4) Sp 2MM 002 (PS4) Sa 2MM 002 (PS4) HF1 2MM 002 (PS4) Hypa 2MM 002 (PS4) HiFi 2MM 002 (PS4) Sp 3MM 002 (PS4) Sa 3MM 002 (PS4) HF1 3MM 002 (PS4) Hypa 3MM 002 (PS4) HiFi 1MM 002 (PS4) Sp 4MM 002 (PS4) Sa 4MM 002 (PS4) HF1 4MM 002 (PS4) Hypa 4MM 002 (PS4) HiFi 4MM 002 (PS4) Sp 5MM 002 (PS4) Sa 5MM 002 (PS4) HF1 5MM 002 (PS4) Hypa 5MM 002 (PS4) HiFi 5MM 002 (PS4) Sp 1MM 003 (EMX1) Sa 1MM 003 (EMX1) HF1 1MM 003 (EMX1) Hypa 1MM 003 (EMX1) HiFi 1MM 003 (EMX1) Sp 2MM 003 (EMX1) Sa 2MM 003 (EMX1) HF1 2MM 003 (EMX1) Hypa 2MM 003 (EMX1) HiFi 2MM 003 (EMX1) Sp 3MM 003 (EMX1) Sa 3MM 003 (EMX1) HF1 3MM 003 (EMX1) Hypa 3MM 003 (EMX1) HiFi 1MM 003 (EMX1) Sp 4MM 003 (EMX1) Sa 4MM 003 (EMX1) HF1 4MM 003 (EMX1) Hypa 4MM 003 (EMX1) HiFi 4MM 003 (EMX1) Sp 5MM 003 (EMX1) Sa 5MM 003 (EMX1) HF1 5MM 003 (EMX1) Hypa 5MM 003 (EMX1) HiFi 5MM 003 (EMX1) base position max Δ abundance Abundance min base position max Δ abundance Abundance min base position max Δ abundance Abundance min base position max Δ abundance Abundance min base position max Δ abundance Abundance min base position max Δ abundance Abundance min base position max Δ abundance Abundance min base position max Δ abundance Abundance min base position max Δ abundance Abundance min base position max Δ abundance Abundance min base position max Δ abundance Abundance min base position max Δ abundance Abundance min base position max Δ abundance Abundance min base position max Δ abundance Abundance min base position max Δ abundance Abundance min base position max Δ abundance Abundance min base position max Δ abundance Abundance min base position max Δ abundance Abundance min base position max Δ abundance Abundance min base position max Δ abundance Abundance min base position max Δ abundance Abundance min base position max Δ abundance Abundance min base position max Δ abundance Abundance min base position max Δ abundance Abundance min base position max Δ abundance Abundance min base position max Δ abundance Abundance min base position max Δ abundance Abundance min base position max Δ abundance Abundance min base position max Δ abundance Abundance min base position max Δ abundance Abundance min base position max Δ abundance Abundance min base position max Δ abundance Abundance min base position max Δ abundance Abundance min base position max Δ abundance Abundance min base position max Δ abundance Abundance min base position max Δ abundance Abundance min base position max Δ abundance Abundance min base position max Δ abundance Abundance min base position max Δ abundance Abundance min base position max Δ abundance Abundance min base position max Δ abundance Abundance min base position max Δ abundance Abundance min base position max Δ abundance Abundance min base position max Δ abundance Abundance min base position max Δ abundance Abundance min base position max Δ abundance Abundance min base position max Δ abundance Abundance min base position max Δ abundance Abundance min base position max Δ abundance Abundance min base position max Δ abundance Abundance min A 20 -0.777718119 0.0248771 A 20 -0.537601265 0.01860604 A 20 -0.145821493 0.098739018 A 20 -0.228016802 0.063862962 A 20 -0.68049449 0.048364975 A 20 -0.73349765 0.041006507 A 20 -0.404108526 0.033797502 A 20 -0.115674007 0.171630017 A 20 -0.19091214 0.089099724 A 20 -0.38256183 0.047098259 A 20 -0.537575803 0.12910201 A 20 -0.262411844 0.104441689 A 20 -0.109184265 0.308311311 A 20 -0.146811895 0.202241092 A 20 -0.194098573 0.155124145 A 20 -0.350743677 0.286842204 A 20 -0.191632203 0.274550599 A 20 -0.110022354 0.494153151 A 20 -0.136951748 0.497139524 A 20 -0.096798856 0.432122617 A 20 -0.201906071 0.564545172 A 20 -0.098027456 0.533106424 A 20 -0.090043424 0.683955588 A 20 -0.092807406 0.733142303 A 20 -0.078883894 0.740609331 A 20 -0.885258429 0.017497841 A 20 -0.310334173 0.181880038 A 20 -0.829762722 0.05502875 A 20 -0.853383445 0.034056135 A 20 -0.809561635 0.020896914 A 20 -0.80691518 0.016557541 A 20 -0.275944098 0.15977429 A 20 -0.747612564 0.044387081 A 20 -0.73131244 0.031054864 A 20 -0.719484341 0.019173452 A 20 -0.659869539 0.096730454 A 20 -0.332178973 0.211131092 A 20 -0.637237248 0.089684622 A 20 -0.511352057 0.076113957 A 20 -0.534849232 0.121716404 A 20 -0.401737967 0.273470945 A 20 -0.213384343 0.377809935 A 20 -0.548573129 0.238520106 A 20 -0.27305229 0.257083934 A 20 -0.254166975 0.316594711 A 20 -0.284599971 0.473719853 A 20 -0.178320044 0.584862618 A 20 -0.539412098 0.323236756 A 20 -0.187580306 0.518051094 A 20 -0.120637388 0.548423798 A 19 -0.894465883 0.022751751 A 19 -0.785993814 0.015464428 A 19 -0.332134972 0.093200781 A 19 -0.497653262 0.044561444 A 19 -0.592572494 0.037416149 A 19 -0.730143114 0.038582704 A 19 -0.532703787 0.036800515 A 19 -0.152197526 0.191667619 A 19 -0.27132605 0.067890669 A 19 -0.365769959 0.051227781 A 19 -0.544589073 0.128454477 A 19 -0.455485753 0.099525232 A 19 -0.144867888 0.299706038 A 19 -0.186508631 0.186849198 A 19 -0.24757387 0.174849462 A 19 -0.386913584 0.290872969 A 19 -0.373158006 0.229638753 A 19 -0.078906321 0.468604985 A 19 -0.13452412 0.442921281 A 19 -0.165775637 0.425581653 A 19 -0.364039211 0.474448845 A 19 -0.360795991 0.409694828 A 19 -0.124477397 0.641795254 A 19 -0.118986563 0.702424911 A 19 -0.141235207 0.663983228 A 19 -0.890144603 0.013995084 A 19 -0.227116304 0.150018865 A 19 -0.755744901 0.049984313 A 19 -0.881806936 0.028725938 A 19 -0.811264213 0.022990006 A 19 -0.796101128 0.015567948 A 19 -0.311293545 0.150748925 A 19 -0.745506059 0.038207301 A 19 -0.752506263 0.026192666 A 19 -0.728393775 0.020686304 A 19 -0.57102033 0.128953857 A 19 -0.227055606 0.221672387 A 19 -0.57055554 0.12725979 A 19 -0.420353405 0.09789689 A 19 -0.433503907 0.141535962 A 19 -0.322300656 0.334075056 A 19 -0.177495969 0.443173816 A 19 -0.503255493 0.291420479 A 19 -0.184430961 0.33333984 A 19 -0.165947506 0.367800465 A 19 -0.148013826 0.608266174 A 19 -0.151243938 0.688452627 A 19 -0.470271023 0.383921984 A 19 -0.099844525 0.666190679 A 19 -0.032633483 0.692371532 A 18 -0.832388815 0.026120194 A 18 -0.721160898 0.017172589 A 18 -0.197460746 0.086791406 A 18 -0.470710873 0.03924049 A 18 -0.740480617 0.031684561 A 18 -0.704246969 0.036125305 A 18 -0.50082525 0.040139319 A 18 -0.177383277 0.154853809 A 18 -0.27752452 0.057672858 A 18 -0.477341128 0.044683556 A 18 -0.530982548 0.125884883 A 18 -0.343031687 0.120461311 A 18 -0.133340205 0.264639661 A 18 -0.184343797 0.181713104 A 18 -0.267406302 0.136167504 A 18 -0.306145222 0.302663835 A 18 -0.272207513 0.291134544 A 18 -0.112137765 0.488448092 A 18 -0.109092453 0.486247751 A 18 -0.167053807 0.41728918 A 18 -0.273097043 0.548454822 A 18 -0.22300959 0.53449674 A 18 -0.098706147 0.692159048 A 18 -0.095548288 0.729504764 A 18 -0.138391339 0.703891557 A 18 -0.856235627 0.024662751 A 18 -0.254906908 0.176371179 A 18 -0.83214007 0.055028959 A 18 -0.858152986 0.025475989 A 18 -0.784535647 0.025881334 A 18 -0.806311389 0.012823463 A 18 -0.314788531 0.163629988 A 18 -0.827038908 0.033194463 A 18 -0.768179087 0.027545073 A 18 -0.71790767 0.018699846 A 18 -0.611237486 0.106096138 A 18 -0.2909173 0.23176211 A 18 -0.657543957 0.085326953 A 18 -0.475860828 0.089752995 A 18 -0.502864987 0.111252503 A 18 -0.324137013 0.316498901 A 18 -0.175836121 0.468978225 A 18 -0.509668002 0.269250958 A 18 -0.209735969 0.369871565 A 18 -0.204997852 0.355407828 A 18 -0.144726444 0.604223847 A 18 -0.164150637 0.712768174 A 18 -0.475612449 0.40038823 A 18 -0.08334648 0.667811951 A 18 -0.035573244 0.676191764 A 17 -0.813431457 0.028576718 A 17 -0.546522304 0.029160141 A 17 -0.25703127 0.099063101 A 17 -0.410930708 0.044130429 A 17 -0.738697126 0.038334811 A 17 -0.714112175 0.038968075 A 17 -0.323385878 0.066988517 A 17 -0.132078812 0.161128415 A 17 -0.228389978 0.076010231 A 17 -0.472665445 0.042071708 A 17 -0.496977886 0.13471758 A 17 -0.244665175 0.19229825 A 17 -0.094005132 0.273328393 A 17 -0.16795317 0.185903071 A 17 -0.23156861 0.142994256 A 17 -0.261846381 0.322954149 A 17 -0.185077816 0.407369239 A 17 -0.080987733 0.499536021 A 17 -0.118925431 0.479626458 A 17 -0.151923214 0.422125444 A 17 -0.201162484 0.601654429 A 17 -0.176675732 0.611568907 A 17 -0.130363059 0.701059586 A 17 -0.10855072 0.753244764 A 17 -0.136740942 0.73691484 A 17 -0.877251053 0.016737846 A 17 -0.35318358 0.199583033 A 17 -0.642380105 0.082030897 A 17 -0.605780486 0.034682723 A 17 -0.786114694 0.018918165 A 17 -0.76638328 0.020230808 A 17 -0.245892795 0.176074215 A 17 -0.59740938 0.053066434 A 17 -0.386136315 0.042005597 A 17 -0.676693572 0.026430721 A 17 -0.532935087 0.131427843 A 17 -0.24480602 0.258649577 A 17 -0.557681634 0.170209887 A 17 -0.234933092 0.165793952 A 17 -0.41829438 0.146009608 A 17 -0.275909032 0.357968834 A 17 -0.144710724 0.474227625 A 17 -0.482963338 0.338267679 A 17 -0.091615714 0.45986849 A 17 -0.109535286 0.416343694 A 17 -0.131011183 0.630474312 A 17 -0.130168377 0.733462062 A 17 -0.487107108 0.385641476 A 17 -0.066759392 0.714231624 A 17 -0.01743768 0.719128805 A 16 -0.684628977 0.03281504 A 16 -0.282047736 0.029396892 A 16 -0.150744504 0.10006683 A 16 -0.116593999 0.060682468 A 16 -0.637596294 0.040835763 A 16 -0.492555435 0.042937119 A 16 -0.267056467 0.12211469 A 16 -0.149692119 0.151310681 A 16 -0.094503542 0.133106075 A 16 -0.328983308 0.053874939 A 16 -0.313166291 0.144037868 A 16 -0.190605524 0.272365335 A 16 -0.110732492 0.283003201 A 16 -0.074246388 0.312060563 A 16 -0.150780853 0.176120707 A 16 -0.191400457 0.378610283 A 16 -0.157340598 0.453761068 A 16 -0.063316751 0.548107237 A 16 -0.06429152 0.612435955 A 16 -0.070765061 0.51894709 A 16 -0.155703637 0.626286374 A 16 -0.188304992 0.676156633 A 16 -0.10329451 0.730911964 A 16 -0.079126242 0.756132227 A 16 -0.097126862 0.776195768 A 16 -0.88060548 0.017285883 A 16 -0.434148266 0.213456447 A 16 -0.764372089 0.049645902 A 16 -0.764255935 0.024800003 A 16 -0.823787169 0.022731821 A 16 -0.759851471 0.014839217 A 16 -0.263496377 0.164940102 A 16 -0.72558034 0.045185213 A 16 -0.552068886 0.03198973 A 16 -0.664152214 0.021105178 A 16 -0.500524432 0.14369847 A 16 -0.178660229 0.278133749 A 16 -0.5283796 0.145509727 A 16 -0.270606529 0.137635824 A 16 -0.357758333 0.144856616 A 16 -0.26046567 0.376856135 A 16 -0.114804815 0.509666454 A 16 -0.461160508 0.327223713 A 16 -0.112581013 0.438483394 A 16 -0.100933611 0.418965154 A 16 -0.10659507 0.678678276 A 16 -0.091565446 0.769561464 A 16 -0.427157189 0.439415692 A 16 -0.046012751 0.785457806 A 16 -0.019935801 0.75581859 A 15 A 15 A 15 A 15 A 15 A 15 A 15 A 15 A 15 A 15 A 15 A 15 A 15 A 15 A 15 A 15 A 15 A 15 A 15 A 15 A 15 A 15 A 15 A 15 A 15 A 15 0 0.017922697 A 15 0 0.176550623 A 15 0 0.074720145 A 15 0 0.036080404 A 15 0 0.024303507 A 15 0 0.021813135 A 15 0 0.165958209 A 15 0 0.073857759 A 15 0 0.070586247 A 15 0 0.026115157 A 15 0 0.134778283 A 15 0 0.281245372 A 15 0 0.192459443 A 15 0 0.21326698 A 15 0 0.15883419 A 15 0 0.34319877 A 15 0 0.488939761 A 15 0 0.342895298 A 15 0 0.481819029 A 15 0 0.404188585 A 15 0 0.592863671 A 15 0 0.685513213 A 15 0 0.37270323 A 15 0 0.684376681 A 15 0 0.669094223 A 14 A 14 A 14 A 14 A 14 A 14 A 14 A 14 A 14 A 14 A 14 A 14 A 14 A 14 A 14 A 14 A 14 A 14 A 14 A 14 A 14 A 14 A 14 A 14 A 14 A 14 0 0.020340852 A 14 0 0.170140738 A 14 0 0.0775036 A 14 0 0.043867007 A 14 0 0.026504102 A 14 0 0.016967642 A 14 0 0.188735439 A 14 0 0.062478657 A 14 0 0.055741194 A 14 0 0.036700293 A 14 0 0.129612996 A 14 0 0.283602619 A 14 0 0.15643891 A 14 0 0.200309204 A 14 0 0.14801799 A 14 0 0.365269593 A 14 0 0.548291304 A 14 0 0.35248665 A 14 0 0.538097347 A 14 0 0.416930943 A 14 0 0.645068242 A 14 0 0.755443176 A 14 0 0.413064958 A 14 0 0.761421503 A 14 0 0.722898563 A 13 -0.559881304 0.053602703 A 13 -0.356242312 0.034178838 A 13 -0.329003375 0.127589494 A 13 -0.069767904 0.134079862 A 13 -0.533718364 0.079177843 A 13 -0.436520364 0.063552838 A 13 -0.311555703 0.114570446 A 13 -0.177137899 0.210635414 A 13 -0.12646342 0.23623047 A 13 -0.278588005 0.097999805 A 13 -0.309936473 0.167740057 A 13 -0.271521175 0.262066616 A 13 -0.107568641 0.379864022 A 13 -0.08851679 0.489794739 A 13 -0.149257854 0.263408149 A 13 -0.20490266 0.384653168 A 13 -0.290690829 0.437465624 A 13 -0.058347326 0.626562685 A 13 -0.078943133 0.744831046 A 13 -0.126956764 0.623565978 A 13 -0.243488458 0.59485151 A 13 -0.25148699 0.615021197 A 13 -0.131658968 0.721216309 A 13 -0.082465655 0.798939726 A 13 -0.144049885 0.747811302 A 13 -0.845002933 0.024314929 A 13 -0.27519296 0.209222874 A 13 -0.772381651 0.070188655 A 13 -0.595388139 0.036715787 A 13 -0.725781878 0.021626211 A 13 -0.754424552 0.020883734 A 13 -0.283604459 0.16668025 A 13 -0.66481454 0.053980495 A 13 -0.369694142 0.050498228 A 13 -0.665897936 0.030834274 A 13 -0.485736823 0.147205833 A 13 -0.114872251 0.273186314 A 13 -0.475838781 0.164650056 A 13 -0.098079773 0.220141359 A 13 -0.325110844 0.154897721 A 13 -0.245529204 0.370698549 A 13 -0.140316304 0.531135433 A 13 -0.452536229 0.351009583 A 13 -0.062644276 0.570446579 A 13 -0.09239346 0.419723025 A 13 -0.099124994 0.68475691 A 13 -0.115835007 0.748303281 A 13 -0.450328585 0.436105577 A 13 -0.038703766 0.83984823 A 13 -0.020977619 0.764433674 A 12 -0.574539839 0.043797631 A 12 -0.305327852 0.047827584 A 12 -0.139703672 0.123563079 A 12 -0.10796843 0.140813388 A 12 -0.45657993 0.060923505 A 12 -0.462997101 0.046557993 A 12 -0.335331775 0.074142944 A 12 -0.106875047 0.196921167 A 12 -0.104498132 0.254748801 A 12 -0.2763558 0.072958844 A 12 -0.329257267 0.158262335 A 12 -0.28848544 0.189211752 A 12 -0.111933879 0.354479914 A 12 -0.124295193 0.484879889 A 12 -0.182881137 0.256433248 A 12 -0.220573696 0.388068715 A 12 -0.249753402 0.409062574 A 12 -0.109710428 0.616768121 A 12 -0.090912513 0.770981339 A 12 -0.128339291 0.631180442 A 12 -0.193848864 0.650002315 A 12 -0.220668105 0.640884162 A 12 -0.10435137 0.782494396 A 12 -0.079156661 0.804506062 A 12 -0.102989384 0.783684311 A 12 -0.882164106 0.020530078 A 12 -0.333027656 0.15041177 A 12 -0.816975425 0.050941552 A 12 -0.870773922 0.028431407 A 12 -0.825073224 0.015548823 A 12 -0.760947916 0.019637716 A 12 -0.232657664 0.169506534 A 12 -0.81231799 0.032274164 A 12 -0.74596582 0.022289363 A 12 -0.657161649 0.025869464 A 12 -0.529439647 0.163572003 A 12 -0.229575093 0.295255731 A 12 -0.622044876 0.113663799 A 12 -0.424085496 0.133037056 A 12 -0.358348574 0.181929752 A 12 -0.269956971 0.397456508 A 12 -0.172255027 0.524852214 A 12 -0.504040895 0.28834459 A 12 -0.176011244 0.4482594 A 12 -0.127024411 0.469178646 A 12 -0.111322247 0.684367033 A 12 -0.105920511 0.725316269 A 12 -0.45523271 0.426082843 A 12 -0.040768301 0.786700956 A 12 -0.01991013 0.762887132 A 11 -0.808436043 0.023975629 A 11 -0.6064773 0.022393141 A 11 -0.544103498 0.087220501 A 11 -0.331936532 0.049127955 A 11 -0.766816985 0.033571312 A 11 -0.72871107 0.043147424 A 11 -0.385333282 0.025380952 A 11 -0.296706668 0.095766406 A 11 -0.209082174 0.087287081 A 11 -0.53891715 0.040796245 A 11 -0.439674916 0.163978484 A 11 -0.267931375 0.127348742 A 11 -0.098304463 0.228919467 A 11 -0.070400097 0.369932495 A 11 -0.223605272 0.211578662 A 11 -0.271869984 0.411281217 A 11 -0.186686549 0.468768586 A 11 -0.077785983 0.597015875 A 11 -0.081727123 0.749828806 A 11 -0.103611251 0.670718324 A 11 -0.131313237 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